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http://hdl.handle.net/10773/7784
Title: | Analysis of the inactivation and reactivation kinetics of horseradish peroxidase in mixtures of phosphate buffer/dimethylformamide |
Author: | Machado, M. F. Saraiva, J. |
Keywords: | Inactivation and reactivation kinetics Horseradish peroxidase Miixtures of phosphate buffer/dimethylformamide |
Issue Date: | 2001 |
Publisher: | Universidade de Aveiro |
Abstract: | In this work, the kinetics of the thermal inactivation and reactivation of horseradish peroxidase were analysed in buffered solutions of dimethylformamide at severa1 concentrations (up to 40% vlv) and temperatures ranging fiom 70 to 85 "C. The experimental data of the inactivation and reactivation processes showed a biphasic behaviour, which was well described by a series-type model. The significant activity regain at low temperature of the thermal inactivated enzyme had a strong influence on the estimated value for the a parameter of the model, thus reflecting a clear change of the profile of the experimental curves. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/7784 |
ISBN: | 972-789-041-5 |
Appears in Collections: | QOPNA - Comunicações |
Files in This Item:
File | Description | Size | Format | |
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5 Proc I 2001 Chempor fatima.pdf | 5.44 MB | Adobe PDF | View/Open |
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