Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/36345
Title: | Development of integrated strategies for the manufacturing of mRNA resorting to aqueous biphasic systems and ionic liquids |
Author: | Costa, Maria I. Freire, Mara G. Silva, Francisca A. e Pedro, Augusto Q. |
Keywords: | mRNA Ionic liquid Integrated manufacturing processes Thermoreversible aqueous biphasic system In vitro transcription T7 RNA polymerase |
Issue Date: | Oct-2022 |
Publisher: | Universidade de Aveiro |
Abstract: | During the COVID-19 pandemic, messenger RNA (mRNA) vaccines appeared as effective approaches to tackle infectious diseases dissemination. However, the manufacturing of mRNA vaccines is complex, costly and resorts to improved technologies to produce high quality and more accessible products. If properly designed, ionic liquids (ILs) can act as RNA stabilizing agents (Pedro et al, ACS Sustain Chem Eng, 6, 2018) and enhance the selectivity of purification processes when used to form aqueous biphasic systems (ABS) (Ventura et al, Chem Rev, 117, 2017). Therefore, and to expedite mRNA production, this work proposes the use of thermoreversible ABS based on ILs to integrate the production and clarification steps, further simplifying subsequent purification steps. Up to date, we have achieved the production of mRNA by in vitro transcription and its purification using conventional methods and gathered insights on mRNA stability and integrity in several structurally distinct ILs. According to these previous results, current attention is being placed on the identification of the best thermoreversible IL-based ABS to integrate production and clarification steps. Overall, the proposed integrated production-clarification platform is expected to tackle current challenges of mRNA manufacturing, especially by improving the cost-efficiency and technological simplicity of existing manufacturing processes and enhancing the stability and yield of the final product. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/36345 |
Publisher Version: | http://jornadasciceco.web.ua.pt/index.html |
Appears in Collections: | CICECO - Comunicações |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Sousa et al_Jornadas CICECO 2022.pdf | 384.44 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.