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http://hdl.handle.net/10773/36127
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DC Field | Value | Language |
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dc.contributor.author | Cardoso, Matias | pt_PT |
dc.contributor.author | Gaspar, Vítor M. | pt_PT |
dc.contributor.author | Ferreira, Carolina | pt_PT |
dc.contributor.author | Silvestre, Ricardo | pt_PT |
dc.contributor.author | Duarte, Iola F. | pt_PT |
dc.contributor.author | Mano, João F. | pt_PT |
dc.date.accessioned | 2023-01-31T10:45:07Z | - |
dc.date.available | 2023-01-31T10:45:07Z | - |
dc.date.issued | 2022-06 | - |
dc.identifier.issn | 1549-9634 | pt_PT |
dc.identifier.uri | http://hdl.handle.net/10773/36127 | - |
dc.description.abstract | This study reports the formulation and delivery of hyaluronic acid-Zein (HA-Zein) nanogels loaded with Shikonin (SK) to selectively attenuate macrophage inflammasome. The self-assembled nanogels, produced by nanoprecipitation, exhibited high encapsulation efficiency, and were selectively internalized by human THP-1-derived macrophages without eliciting cytotoxic responses. Cell treatment with HA-Zein-SK nanogels before stimulation with LPS and Nigericin significantly suppressed caspase-1 activation and IL-1β production, indicating inflammasome inhibition. Importantly, HA-Zein-SK nanogels bioinstructed inflammasome activated macrophages towards an anti-inflammatory CD163highHLA-DRlow phenotype and led to a marked reduction in the release of pro-inflammatory mediators (TNF-α, IL-6 and IP-10). Extracellular metabolic profiling additionally revealed SK-mediated downregulation of cellular glycolytic activity, which was corroborated by a significant decrease of glycolytic genes transcription. All in all, our findings demonstrate the potential of bioactive SK-containing, self-assembled nanogels to modulate exacerbated responses in innate immune cells and, prospectively, in human tissues where NRLP3 inflammasome is abnormally activated upon injury or disease. | pt_PT |
dc.language.iso | eng | pt_PT |
dc.publisher | Elsevier | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50011%2F2020/PT | pt_PT |
dc.relation | LA/P/0006/2020 | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50011%2F2020/PT | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F139539%2F2018/PT | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/PD%2FBDE%2F127830%2F2016/PT | pt_PT |
dc.relation | CEECIND/00185/2020 | pt_PT |
dc.relation | CEECIND/1048/2019 | pt_PT |
dc.rights | restrictedAccess | pt_PT |
dc.subject | Interleukin-1beta | pt_PT |
dc.subject | Macrophages | pt_PT |
dc.subject | NLR Family, Pyrin Domain-Containing 3 Protein | pt_PT |
dc.subject | Nanogels | pt_PT |
dc.subject | Naphthoquinones | pt_PT |
dc.subject | Inflammasomes | pt_PT |
dc.subject | Zein | pt_PT |
dc.title | Macrophage-targeted shikonin-loaded nanogels for modulation of inflammasome activation | pt_PT |
dc.type | article | pt_PT |
dc.description.version | published | pt_PT |
dc.peerreviewed | yes | pt_PT |
degois.publication.title | Nanomedicine: Nanotechnology, Biology, and Medicine | pt_PT |
degois.publication.volume | 42 | pt_PT |
dc.identifier.doi | 10.1016/j.nano.2022.102548 | pt_PT |
dc.identifier.essn | 1549-9642 | pt_PT |
dc.identifier.articlenumber | 102548 | pt_PT |
Appears in Collections: | CICECO - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Cardoso2022_Nanomedicine.pdf | 3.07 MB | Adobe PDF |
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