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Title: Protein cohabitation: Improving the photochemical stability of R-Phycoerythrin in the solid state
Author: Bharmoria, Pankaj
Correia, Sandra F. H.
Martins, Margarida
Hernández-Rodríguez, Miguel A.
Ventura, Sónia P. M.
Ferreira, Rute A. S.
Carlos, Luís D.
Coutinho, João A. P.
Issue Date: 6-Aug-2020
Publisher: American Chemical Society
Abstract: The poor photochemical stability of R-phycoerythrin (R-PE) has been a bottleneck for its broad-spectrum applications. Inspired by nature, we studied a sustainable strategy of protein cohabitation to enhance R-PE stability by embedding it in a solid matrix of gelatin. Both pure R-PE and fresh phycobiliprotein (PBP) extracts recovered from Gracilaria gracilis were studied. The incorporation of R-PE in the gelatin-based films (gelatin-RPE and gelatin-PBPs) has improved its photochemical stability for at least 8 months, the longest time period reported so far. These results were evidenced by not only absorption but also emission quantum yield measurements (Φ). Moreover, the photostability of gelatin-RPE films upon continuous excitation with an AM1.5G solar simulator was tested and found to remain stable for 23 h after initial decreasing up to 250 min. In the end, another approach was established to allow 100% photostability for a 3 h exposure to an AM1.5G solar simulator by doping the gelatin-based film including R-Phycoerythrin with n-propyl gallate stabilized with Tween 80, allowing their use as naturally based optically active centers in photovoltaic applications.
Peer review: yes
DOI: 10.1021/acs.jpclett.0c01491
ISSN: 1948-7185
Appears in Collections:CICECO - Artigos

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