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http://hdl.handle.net/10773/30202| Title: | Continuous separation of cytochrome-c PEGylated conjugates by fast centrifugal partition chromatography |
| Author: | Santos, João H. P. M. Ferreira, Ana M. C. Almeida, Mafalda R. Quinteiro, Paula S. G. N. Dias, Ana C. R. V. Coutinho, João A. P. Freire, Mara G. Rangel-Yagui, Carlota O. Ventura, Sónia P. M. |
| Issue Date: | 2019 |
| Publisher: | Royal Society of Chemistry |
| Abstract: | Herein, the effective use of aqueous biphasic systems (ABS) in Fast Centrifugal Partition Chromatography (FCPC) for the purification of PEGylated cytochrome c conjugates is shown. High recoveries (between 88% and 100%) and purities (∼100%) were obtained. Both the unreacted cytochrome c and solvents may be recovered and reused, thus allowing the design of a sustainable process in a continuous regime for the isolation of bioconjugates. This process allowed the reduction of the complete E-factor and carbon footprint at circa 100% and 67%, respectively, reinforcing the important environmental contribution of recycling units. |
| Peer review: | yes |
| URI: | http://hdl.handle.net/10773/30202 |
| DOI: | 10.1039/C9GC01063G |
| ISSN: | 1463-9262 |
| Appears in Collections: | CESAM - Artigos CICECO - Artigos |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| c9gc01063g.pdf | 2.02 MB | Adobe PDF |  |
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