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Title: Continuous separation of cytochrome-c PEGylated conjugates by fast centrifugal partition chromatography
Author: Santos, João H. P. M.
Ferreira, Ana M. C.
Almeida, Mafalda R.
Quinteiro, Paula S. G. N.
Dias, Ana C. R. V.
Coutinho, João A. P.
Freire, Mara G.
Rangel-Yagui, Carlota O.
Ventura, Sónia P. M.
Issue Date: 2019
Publisher: Royal Society of Chemistry
Abstract: Herein, the effective use of aqueous biphasic systems (ABS) in Fast Centrifugal Partition Chromatography (FCPC) for the purification of PEGylated cytochrome c conjugates is shown. High recoveries (between 88% and 100%) and purities (∼100%) were obtained. Both the unreacted cytochrome c and solvents may be recovered and reused, thus allowing the design of a sustainable process in a continuous regime for the isolation of bioconjugates. This process allowed the reduction of the complete E-factor and carbon footprint at circa 100% and 67%, respectively, reinforcing the important environmental contribution of recycling units.
Peer review: yes
DOI: 10.1039/C9GC01063G
ISSN: 1463-9262
Appears in Collections:CESAM - Artigos
CICECO - Artigos

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