Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/29317
Title: In vivo interaction of the hepatitis delta virus small antigen with the ELAV-like protein HuR
Author: Casaca, Ana
Fardilha, Margarida
Silva, Edgar da Cruz e
Cunha, Celso
Keywords: Hepatitis delta virus
Delta antigen
Yeast two-hybrid
HuR
Issue Date: 2011
Publisher: Bentham Open
Abstract: The small and large delta antigens (S-HDAg and L-HDAg, respectively) represent two forms of the only protein encoded by the hepatitis delta virus (HDV) RNA genome. Consequently, HDV relies, at a large extent, on the host cell machinery for replication and transcription. Until now, only a limited number of cellular proteins were identified as S-HDAg or L-HDAg partners being involved in the modulation of the virus life cycle. In an attempt to identify cellular S-HDAg-binding proteins we made use of a yeast two-hybrid approach to screen a human liver cDNA library. We were able to identify HuR, a ubiquitously expressed protein involved in RNA stabilization, as an S-HDAg partner both in vitro and in vivo. HuR was found to be overexpressed and colocalize with HDAg in human hepatoma cells. siRNA knockdown of HuR mRNA resulted in inhibition of S-HDAg and L-HDAg expression.
Peer review: yes
URI: http://hdl.handle.net/10773/29317
DOI: 10.2174/1874357901105010012
ISSN: 1874-3579
Appears in Collections:CBC - Artigos

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