Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorSousa, Bárbara M. dept_PT
dc.contributor.authorCorreia, Patrícia D.pt_PT
dc.contributor.authorChato-Astraín, Jesúspt_PT
dc.contributor.authorSilva, Raquelpt_PT
dc.contributor.authorMüller, Hans Wernerpt_PT
dc.contributor.authorCarriel, Víctorpt_PT
dc.contributor.authorBosse, Frankpt_PT
dc.contributor.authorVieira, Sandra I.pt_PT
dc.description.abstractSpinal cord injury (SCI) seriously impairs functions below injury level and has no effective treatment yet. Central neurons may be stimulated to regenerate in more permissive environments. Our mining for transcriptomic data from manipulated rodent SCI models presenting improved motor functions, retrieved sets of common differentially expressed genes (DEGs) involved in recovery. Amongst the up-regulated DEGs was KIF4A, an anterograde kinesin essential throughout development but downregulated postnatally. KIF4A is unaltered in the spinal cord of untreated SCI animals at the acute phase. This work aimed to characterize KIF4A levels and distribution in various PNS injury (PNI) regenerative rodent models. KIF4A signal was analysed by IHC(F) and qRT-PCR, in DRGs and distal nerve stumps, upon two PNI lesions (sciatic crush versus the less regenerative transection). KIF4A distribution was also studied in spinal cord tissues upon sciatic nerve transection. qRT-PCR revealed that KIF4A expression in sciatic nerve and DRGs is upregulated upon both lesion paradigms (2dpi). At 7dpi, KIF4A levels at the distal stump increase more than 15x. In both DRGs and distal stumps, KIF4A is particularly upregulated in the spontaneously regenerative crush paradigm. IHC(F) revealed basal KIF4A distribution in axons, DRGs neurons’ nuclei and Schwann cells. Following sciatic transection, KIF4A was observed in motor neurons’ nuclei at the spinal cord region to where injured sciatic nerve projects. Transcriptomic data mining and experimental data relate KIF4A levels with neuronal regeneration, where nuclear KIF4A may have anti-apoptotic roles. Subsequent functional assays must be addressed following modulation of KIF4A levels.pt_PT
dc.subjectSpinal cord injurypt_PT
dc.subjectPeripheral nerve injurypt_PT
dc.titleKIF4A: unravelling a potential spinal cord repair gene using regenerative peripheral nerve injury modelspt_PT
ua.event.date11-15 julho, 2020pt_PT
degois.publication.titleFENS Virtual Forum 2020pt_PT
Appears in Collections:IBIMED - Comunicações

Files in This Item:
File Description SizeFormat 
FENS Poster - KIF4A - Final.pdf2.24 MBAdobe PDFrestrictedAccess

Formato BibTex MendeleyEndnote Degois 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.