Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/27821
Title: | Conserved and highly expressed tRNA derived fragments in zebrafish |
Author: | Soares, Ana Raquel Fernandes, Noémia Reverendo, Marisa Araújo, Hugo Rafael Oliveira, José Luís Moura, Gabriela M. R. Santos, Manuel A. S. |
Keywords: | tRNA derived fragments Zebrafish Small non coding RNAs tRNAs |
Issue Date: | 22-Dec-2015 |
Abstract: | Background: Small non-coding RNAs (sncRNAs) are a class of transcripts implicated in several eukaryotic regulatory mechanisms, namely gene silencing and chromatin regulation. Despite significant progress in their identification by next generation sequencing (NGS) we are still far from understanding their full diversity and functional repertoire. Results: Here we report the identification of tRNA derived fragments (tRFs) by NGS of the sncRNA fraction of zebrafish. The tRFs identified are 18–30 nt long, are derived from specific 5′ and 3′ processing of mature tRNAs and are differentially expressed during development and in differentiated tissues, suggesting that they are likely produced by specific processing rather than random degradation of tRNAs. We further show that a highly expressed tRF (5′tRF-ProCGG) is cleaved in vitro by Dicer and has silencing ability, indicating that it can enter the RNAi pathway. A computational analysis of zebrafish tRFs shows that they are conserved among vertebrates and mining of publicly available datasets reveals that some 5′tRFs are differentially expressed in disease conditions, namely during infection and colorectal cancer. Conclusions: tRFs constitute a class of conserved regulatory RNAs in vertebrates and may be involved in mechanisms of genome regulation and in some diseases. Keywords: tRNA derived fragments, Zebrafish, Small non coding RNAs, tRNAs |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/27821 |
DOI: | 10.1186/s12867-015-0050-8 |
Appears in Collections: | IBIMED - Artigos IEETA - Artigos DCM - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Soares et al. 2015.pdf | 2.39 MB | Adobe PDF | View/Open |
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