Please use this identifier to cite or link to this item:
Title: Hybrid alginate-protein cryogel beads: efficient and sustainable bio-based materials to purify immunoglobulin G antibodies
Author: Sharma, Mukesh
Tavares, Ana Paula Mora
Nunes, João C. F.
Singh, Nripat
Mondal, Dibyendu
Neves, Márcia C.
Prasad, Kamalesh
Freire, Mara G.
Issue Date: Mar-2020
Publisher: Royal Society of Chemistry
Abstract: Antibodies present in mammal’s serum are of high relevance for therapeutic purposes, particularly in passive immunization and in the treatment of some chronic diseases. However, their recovery with high purity and yield is still compromised by the requirement of several process steps and constraint of keeping antibodies stable to not compromise their therapeutic efficiency. These challenges significantly contribute to the current high-cost of biopharmaceuticals, namely antibodies such as immunoglobulin G (IgG). Accordingly, the development of effective and sustainable purification strategies for antibodies and other biopharmaceuticals is in critical demand, while allowing to decrease economic, environmental and health cargos. Herein, bio-based and low-cost hybrid alginate-protein cryogel beads were prepared, characterized, and applied as novel adsorbent materials for the purification of IgG from human serum. It is shown that hybrid materials are more efficient than the respective alginate beads since the presence of proteins increases the materials selectivity for IgG. Several operating conditions, such as pH, adsorption time and serum concentration, were optimized to improve the recovery yield and purity of IgG. Adsorption isotherms were also determined to infer the adsorption mechanism of IgG onto the cryogel beads and to determine their maximum adsorption capacity (175 mg of IgG per g of cryogel beads). At the optimized conditions, IgG can be recovered from the hybrid materials using buffered aqueous solutions, with a purity of 80% and a recovery yield of 91%. The stability and integrity of the antibody is kept after the desorption step. Finally, the regeneration and reuse of the cryogel beads was evaluated, with no losses on the IgG adsorption performance and antibody stability. Although significant efforts have been placed on the development of novel affinity ligands to replace the standard chromatographic methods to purify IgG, this works demonstrates the potential of bio-based and low-cost hybrid materials as promising alternatives, in which proteins can be used to improve the materials selectivity.
Peer review: yes
DOI: 10.1039/C9GC04449C
ISSN: 1463-9262
Appears in Collections:CICECO - Artigos

Files in This Item:
File Description SizeFormat 
ESI.docx105.72 kBMicrosoft Word XMLView/Open
c9gc04449c.pdf1.47 MBAdobe PDFView/Open

Formato BibTex MendeleyEndnote Degois 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.