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http://hdl.handle.net/10773/20170
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DC Field | Value | Language |
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dc.contributor.author | Melo, Tania | pt |
dc.contributor.author | Domingues, Pedro | pt |
dc.contributor.author | Ferreira, Rita | pt |
dc.contributor.author | Milic, Ivana | pt |
dc.contributor.author | Fedorova, Maria | pt |
dc.contributor.author | Santos, Sergio M. | pt |
dc.contributor.author | Segundo, Marcela A. | pt |
dc.contributor.author | Domingues, M. Rosario M. | pt |
dc.date.accessioned | 2017-12-07T19:37:52Z | - |
dc.date.issued | 2016 | pt |
dc.identifier.issn | 0003-2700 | pt |
dc.identifier.uri | http://hdl.handle.net/10773/20170 | - |
dc.description.abstract | In recent years, there has been an increasing interest in nitro fatty acids (NO2-FA) as signaling molecules formed under nitroxidative stress. NO2-FA were detected in vivo in a free form, although it is assumed that they may also be esterified to phospholipids (PL). Nevertheless, insufficient discussion about the nature, origin, or role of nitro phospholipids (NO2-PL) was reported up to now. The aim of this study was to develop a mass spectrometry (MS) based approach which allows identifying nitroalkenes derivatives of three major PL classes found in living systems: phosphatidylcholines (PCs), phosphatidylethanolamine (PEs), and phosphatidylserines (PSs). NO2-PLs were generated by NO2BF4 in hydrophobic environment, mimicking biological systems. The NO2-PLs were then detected by electrospray ionization (ESI-MS) and ESI-MS coupled to hydrophilic interaction liquid chromatography (HILIC). Identified NO2-PLs were further analyzed by tandem MS in positive (as [M + H](+) ions for all PL classes) and negative-ion mode (as [M - H](-) ions for PEs and PSs and [M + OAc](-) ions for PCs). Typical MS/MS fragmentation pattern of all NO2-PL included a neutral loss of HNO2, product ions arising from the combined loss of polar headgroup and HNO2, [NO2-FA + H](+) and [NO2-FA - H](-) product ions, and cleavages on the fatty acid backbone near the nitro group, allowing its localization within the FA akyl chain. Developed MS method was used to identify NO2-PL in cardiac mitochondria from a well-characterized animal model of type 1 diabetes mellitus. We identified nine NO2-PCs and one NO2-PE species. The physiological relevance of these findings is still unknown. | pt |
dc.language.iso | eng | pt |
dc.publisher | AMER CHEMICAL SOC | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147415/PT | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147258/PT | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F84691%2F2012/PT | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147332/PT | pt |
dc.rights | restrictedAccess | por |
dc.subject | LINOLEIC-ACID NITRATION | pt |
dc.subject | NITRIC-OXIDE | pt |
dc.subject | FATTY-ACIDS | pt |
dc.subject | ANTIINFLAMMATORY PROPERTIES | pt |
dc.subject | STRUCTURAL-CHARACTERIZATION | pt |
dc.subject | ELECTROSPRAY-IONIZATION | pt |
dc.subject | HUMAN BLOOD | pt |
dc.subject | OLEIC-ACID | pt |
dc.subject | FRAGMENTATION | pt |
dc.subject | PEROXYNITRITE | pt |
dc.title | Recent Advances on Mass Spectrometry Analysis of Nitrated Phospholipids | pt |
dc.type | article | pt |
dc.peerreviewed | yes | pt |
ua.distribution | international | pt |
degois.publication.firstPage | 2622 | pt |
degois.publication.issue | 5 | pt |
degois.publication.lastPage | 2629 | pt |
degois.publication.title | ANALYTICAL CHEMISTRY | pt |
degois.publication.volume | 88 | pt |
dc.date.embargo | 10000-01-01 | - |
dc.relation.publisherversion | 10.1021/acs.analchem.5b03407 | pt |
dc.identifier.doi | 10.1021/acs.analchem.5b03407 | pt |
Appears in Collections: | CICECO - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Recent Advances on Mass Spectrometry Analysis of Nitrated Phospholipids_10.1021acs.analchem.5b03407.pdf | 1.34 MB | Adobe PDF |
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