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http://hdl.handle.net/10773/19804
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DC Field | Value | Language |
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dc.contributor.author | Silva, Nuno H. C. S. | pt |
dc.contributor.author | Pinto, Ricardo J. B. | pt |
dc.contributor.author | Freire, Carmen S. R. | pt |
dc.contributor.author | Marrucho, Isabel M. | pt |
dc.date.accessioned | 2017-12-07T19:25:24Z | - |
dc.date.issued | 2016 | pt |
dc.identifier.issn | 0927-7765 | pt |
dc.identifier.uri | http://hdl.handle.net/10773/19804 | - |
dc.description.abstract | Amyloid fibrils have recently gained a lot of attention due to their morphology, functionality and mechanical strength, allowing for their application in nanofiber-based materials, biosensors, bioactive membranes and tissue engineering scaffolds. The in vitro production of amyloid fibrils is still a slow process, thus hampering the massive production of nanofibers and its consequent use. This work presents a new and faster (2-3 h) fibrillation method for hen egg white lysozyme (HEWL) using a deep eutectic solvent based on cholinium chloride and acetic acid. Nanofibers with dimensions of 0.5-1 mu m in length and 0.02-0.1 mu m in thickness were obtained. Experimental variables such as temperature and pH were also studied, unveiling their influence in fibrillation time and nanofibers morphology. These results open a new scope for protein fibrillation into nanofibers with applications ranging from medicine to soft matter and nanotechnology. (C) 2016 Elsevier B.V. All rights reserved. | pt |
dc.language.iso | eng | pt |
dc.publisher | ELSEVIER SCIENCE BV | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F85690%2F2012/PT | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F89982%2F2012/PT | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147332/PT | pt |
dc.rights | restrictedAccess | por |
dc.subject | EGG-WHITE LYSOZYME | pt |
dc.subject | TRANSITION-TEMPERATURE MIXTURES | pt |
dc.subject | AMYLOID FIBRIL FORMATION | pt |
dc.subject | HUMAN SERUM-ALBUMIN | pt |
dc.subject | IONIC LIQUIDS | pt |
dc.subject | CHOLINE CHLORIDE | pt |
dc.subject | CARBOXYLIC-ACIDS | pt |
dc.subject | ALPHA-SYNUCLEIN | pt |
dc.subject | HEN LYSOZYME | pt |
dc.subject | X-RAY | pt |
dc.title | Production of lysozyme nanofibers using deep eutectic solvent aqueous solutions | pt |
dc.type | article | pt |
dc.peerreviewed | yes | pt |
ua.distribution | international | pt |
degois.publication.firstPage | 36 | pt |
degois.publication.lastPage | 44 | pt |
degois.publication.title | COLLOIDS AND SURFACES B-BIOINTERFACES | pt |
degois.publication.volume | 147 | pt |
dc.date.embargo | 10000-01-01 | - |
dc.relation.publisherversion | 10.1016/j.colsurfb.2016.07.005 | pt |
dc.identifier.doi | 10.1016/j.colsurfb.2016.07.005 | pt |
Appears in Collections: | CICECO - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Production of lysozyme nanofibers using deep eutectic solvent aqueous solutions_10.1016j.colsurfb.2016.07.005.pdf | 1.2 MB | Adobe PDF |
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