Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/19657
Title: Insights into the impact of silver nanoparticles on human keratinocytes metabolism through NMR metabolomics
Author: Carrola, Joana
Bastos, Veronica
Ferreira de Oliveira, Jose Miguel P.
Oliveira, Helena
Santos, Conceicao
Gil, Ana M.
Duarte, Iola F.
Keywords: IN-VITRO
SILICA NANOPARTICLES
CELL-CULTURE
LIPID RAFTS
TOXICITY
CYTOTOXICITY
NANOMATERIALS
H-1-NMR
LINE
SKIN
Issue Date: 2016
Publisher: ELSEVIER SCIENCE INC
Abstract: Due to their antimicrobial properties, silver nanoparticles (AgNPs) are increasingly incorporated into consumer goods and medical products. Their potential toxicity to human cells is however a major concern, and there is a need for improved understanding of their effects on cell metabolism and function. Here, Nuclear Magnetic Resonance (NMR) metabolomics was used to investigate the metabolic profile of human epidermis keratinocytes (HaCaT cell line) exposed for 48 h to 30 nm citrate-stabilized spherical AgNPs (10 and 40 p.g/mL). Intracellular aqueous extracts, organic extracts and extracellular culture medium were analysed to provide an integrated view of the cellular metabolic response. The specific metabolite variations, highlighted through multivariate analysis and confirmed by spectral integration, suggested that HaCaT cells exposed to AgNPs displayed upregulated glutathione-based antioxidant protection, increased glutaminolysis, downregulated tricarboxylic acid (TCA) cycle activity, energy depletion and cell membrane modification. Importantly, most metabolic changes were apparent in cells exposed to a concentration of AgNPs which did not affect cell viability at significant levels, thus underlying the sensitivity of NMR metabolomics to detect early biochemical events, even in the absence of a clear cytotoxic response. It can be concluded that NMR metabolomics is an important new tool in the field of in vitro nanotoxicology. (C) 2015 Elsevier Inc. All rights reserved.
Peer review: yes
URI: http://hdl.handle.net/10773/19657
DOI: 10.1016/j.abb.2015.08.022
ISSN: 0003-9861
Publisher Version: 10.1016/j.abb.2015.08.022
Appears in Collections:CICECO - Artigos



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