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http://hdl.handle.net/10773/19201
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DC Field | Value | Language |
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dc.contributor.author | Gupta, Bhupender S. | pt |
dc.contributor.author | Taha, Mohamed | pt |
dc.contributor.author | Lee, Ming-Jer | pt |
dc.date.accessioned | 2017-12-07T19:04:26Z | - |
dc.date.available | 2017-12-07T19:04:26Z | - |
dc.date.issued | 2016 | pt |
dc.identifier.issn | 2046-2069 | pt |
dc.identifier.uri | http://hdl.handle.net/10773/19201 | - |
dc.description.abstract | In this work, the extraction of a model enzyme alpha-chymotrypsin in its active conformation from an aqueous solution by using new biocompatible and self-buffering Good's buffer ionic liquids (GBILs)-based aqueous biphasic systems (ABSs) is demonstrated. The experimental liquid-liquid phase boundary data for these new GBIL-based ABSs were measured at 298.2 K. These new ionic liquids are derived from biological Good's buffers as anions and tetra-alkylammonium as cations. The buffering potential of these GBILs was confirmed by measuring their pH profiles and protonation constants (pK(a)) in aqueous solution by a potentiometric method at various temperatures. Moreover, these GBILs provide a greater stabilizing effect on the activity of alpha-chymotrypsin, as compared with the corresponding Good's buffers. Therefore, the new self-buffering GBILs may not only serve as a biocompatible medium for enzymatic research, but also avoid the use of an external buffering agent, which is often needed for pH control in the case of using a conventional ILs-based medium. Therefore, these new biocompatible GBILs are more advantageous over the conventional ILs-based media for enzymatic research. | pt |
dc.language.iso | eng | pt |
dc.publisher | ROYAL SOC CHEMISTRY | pt |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147332/PT | pt |
dc.rights | openAccess | por |
dc.subject | AQUEOUS BIPHASIC SYSTEMS | pt |
dc.subject | ALPHA-CHYMOTRYPSIN | pt |
dc.subject | BIOLOGICAL BUFFERS | pt |
dc.subject | DIRECT ELECTROCHEMISTRY | pt |
dc.subject | CARBON NANOTUBES | pt |
dc.subject | PH BUFFERS | pt |
dc.subject | SOLVENTS | pt |
dc.subject | CATALYSIS | pt |
dc.subject | AGGREGATION | pt |
dc.subject | EQUILIBRIA | pt |
dc.title | Extraction of an active enzyme by self-buffering ionic liquids: a green medium for enzymatic research | pt |
dc.type | article | pt |
dc.peerreviewed | yes | pt |
ua.distribution | international | pt |
degois.publication.firstPage | 18567 | pt |
degois.publication.issue | 22 | pt |
degois.publication.lastPage | 18576 | pt |
degois.publication.title | RSC ADVANCES | pt |
degois.publication.volume | 6 | pt |
dc.relation.publisherversion | 10.1039/c6ra00607h | pt |
dc.identifier.doi | 10.1039/c6ra00607h | pt |
Appears in Collections: | CICECO - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Extraction of an active enzyme by self-buffering ionic liquids A green medium for enzymatic research_10.1039c6ra00607h.pdf | 728.33 kB | Adobe PDF | View/Open |
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