Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/11064
Title: | Tetracycline-resistance genes in Gram-negative isolates from estuarine waters |
Author: | Henriques, I. S. Fonseca, F. Alves, A. Saavedra, M. J. Correia, A. |
Keywords: | Aeromonas antimicrobial resistance Enterobacteriaceae estuarine waters gene transfer |
Issue Date: | 2008 |
Publisher: | Wiley-Blackwell |
Abstract: | Forty-two out of 164 multi-resistant isolates previously obtained were resistant or less-susceptible to tetracycline, as evaluated by the disc diffusion method. Minimal inhibitory concentration for resistant bacteria ranged from 16 to 256 mg l−1. Screening of tet genes by polymerase chain reaction showed that 88% of the isolates carried at least one of the genes tested, namely tet(A) (present in 13 isolates), tet(B) (present in 13 isolates), tet(C) (present in 3 isolates), tet(D) (present in 1 isolate), tet(E) (present in 6 isolates) and tet(M) (present in 1 isolate). One isolate carried tet(A) and tet(M). To our knowledge, this study presents the first description of a tet(D) gene in Morganella morganii. Hybridization revealed that tet genes were plasmid-located in 31% of the isolates. Those isolates were included as donors in conjugation experiments and 38% transferred tetracycline resistance. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/11064 |
DOI: | 10.1111/j.1472-765X.2008.02452.x |
ISSN: | 0266-8254 |
Appears in Collections: | PT Mar - Artigos Ria de Aveiro - Artigos |
Files in This Item:
File | Description | Size | Format | |
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165_Tetracycline-resistance genes.pdf | 111.49 kB | Adobe PDF |
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