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http://hdl.handle.net/10773/7036
Title: | Thermal stability and activity regain of horseradish peroxidase in aqueous mixtures of imidazolium-based ionic liquids |
Author: | Machado, Maria Fátima Saraiva, Jorge Manuel |
Keywords: | Activity regain Horseradish peroxidase Ionic liquids Kinetic parameters Thermal deactivation |
Issue Date: | 2005 |
Publisher: | Springer Verlag |
Abstract: | Thermal deactivation kinetics of horseradish peroxidase (HRP) were studied from 45 to 90 degrees C in phosphate buffer and 5-25% (v,w/v) 1-butyl-3-methylimidazolium tetrafluoroborate [BMIM][BF4] and 1-butyl-3-methylimidazolium chloride [BMIM][Cl]. HRP activity at 25 degrees C was not affected by the presence of ionic liquids up to 20% (v,w/v). Increasing the ionic liquids concentration up to 25% (v,w/v) changed the biphasic character of deactivation kinetics to an apparent single first-order step. The presence of 5-10% (v/v) [BMIM][BF4] significantly improved HRP thermal stability with lower activation energies for the deactivation second phase (83-87 kJ mol(-1)). After deactivation, enhanced activity regain of the enzyme, up to 70-80% of the initial activity, was found in 25% (v/v) [BMIM][BF4] and 10% (w/v) [BMIM][Cl] and correlated to prevalence of the deactivation first phase. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/7036 |
DOI: | 10.1007/s10529-005-0023-y |
ISSN: | 0141-5492 |
Appears in Collections: | QOPNA - Artigos |
Files in This Item:
File | Description | Size | Format | |
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P13_BL_ Peroxidase IL.pdf | 263.87 kB | Adobe PDF |
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