Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/6965
Title: | High pressure treatment as a tool for engineering of enzymatic reactions in cellulosic fibres |
Author: | Oliveira, Sandra C. T. Figueiredo, Andreia B. Evtuguin, Dmitry V. Saraiva, Jorge A. |
Keywords: | Kraft pulp Xylan High hydrostatic pressure treatment Enzymatic hydrolysis Xylanase |
Issue Date: | 2012 |
Publisher: | Elsevier |
Abstract: | Ultrahigh hydrostatic pressure (UHP) was applied to enhance the enzymatic hydrolysis of xylan in cellulosic fibers. The pretreatment improved xylan accessibility in the fiber cell wall and a 5-10-fold increase in the initial hydrolysis rate of xylan by xylanase was observed when Eucalyptus globulus kraft bleached pulp was pretreated at hydrostatic pressures of 300-400MPa for 15-45min. The rate and the extent of hydrolysis can be controlled by varying the pressure and the exposure time of UHP treatment prior to enzymatic hydrolysis. Sequential UHP pretreatment and hydrolysis by xylanase disaggregated the hydrated cellulose fibrils and led to the gelation of cell wall regions accessible to enzymatic attack. UHP could therefore become a tool for the engineering of enzymatic reactions within cellulosic fibers to obtain products with tailored properties. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/6965 |
DOI: | 10.1016/j.biortech.2011.12.093 |
ISSN: | 0960-8524 |
Appears in Collections: | QOPNA - Artigos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
P44_Biores TEchn_HP_Cellulose.pdf | 363.1 kB | Adobe PDF |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.