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http://hdl.handle.net/10773/5106| Title: | Analysis of linoleic acid hydroperoxides generated by biomimetic and enzymatic systems through an integrated methodology |
| Author: | Villaverde, Juan José Santos, Sónia A.O. Simões, Mário M.Q. Neto, Carlos Pascoal Domingues, M. Rosário M. Silvestre, Armando J.D. |
| Keywords: | Oxidation Linoleic acid Linoleic acid hydroperoxides Isomers HPLC–UV HPLC–MS/MS |
| Issue Date: | 2011 |
| Publisher: | Elsevier |
| Abstract: | An integrated methodology for the identification and quantification of linoleic acid hydroperoxides (HPODEs) mixtures, obtained by the Fenton's reaction and by enzymatic oxidation, is reported. Unambiguous identification of the HPODEs formed (13-hydroperoxy-(9Z,11E)-octadecadienoic acid, 13-hydroperoxy-(9E,11E)-octadecadienoic acid and 9-hydroperoxyoctadecadienoic acid) was performed by HPLC-MS/MS analysis, while quantification was carried out by HPLC-UV using an external calibration, based on the collection of chromatographic peaks, and measuring of total hydroperoxides by ferrous oxidation-xylenol orange (FOX) method. (C) 2011 Elsevier B.V. All rights reserved. |
| Peer review: | yes |
| URI: | http://hdl.handle.net/10773/5106 |
| DOI: | 10.1016/j.indcrop.2011.05.001 |
| ISSN: | 0926-6690 |
| Appears in Collections: | DQ - Artigos |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| industrial crops and products 34 (2011)1474_81.pdf | 995.7 kB | Adobe PDF |  |
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