Utilize este identificador para referenciar este registo:
http://hdl.handle.net/10773/17725
Título: | Control of cell alignment and morphology by redesigning ECM-mimetic nanotopography on multilayer membranes |
Autor: | Sousa, Maria P. Caridade, Sofia G. Mano, João F. |
Palavras-chave: | Layer-by- layer Polysaccharides Nanogrooved freestanding membranes Cell alignment C2C12 differentiation |
Data: | 29-Mar-2017 |
Editora: | Wiley |
Resumo: | Inspired by native extracellular matrix (ECM) together with the multilevel architecture observed in nature, we engineer a material which topography recapitulates topographic features of the ECM and the internal architecture mimics the biological materials organization. The nanopatterned design along the XY plane is combined with a nanostructured organization along the Z axis on freestanding membranes prepared by layer-by-layer deposition of chitosan and chondroitin sulfate. Cellular behavior is monitored using two different mammalian cell lines, fibroblasts (L929) and myoblasts (C2C12), in order to perceive the response to topography. Viability, proliferation and morphology of L929 are sensitively controlled by topography; also differentiation of C2C12 into myotubes is influenced by the presence of nanogrooves. This kind of nanopatterned structure has been also associated with strong cellular alignment. To the best of our knowledge, it is the first time that such a straightforward and inexpensive strategy is proposed to produce nanopatterned freestanding multilayer membranes. Controlling cellular alignment plays a critical role in many human tissues, such as muscles, nerves or blood vessels, so these membranes can be potentially useful in specific tissue regeneration strategies. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/17725 |
DOI: | 10.1002/adhm.201601462 |
ISSN: | 2192-2659 |
Aparece nas coleções: | CICECO - Artigos |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Sousa etal_Proofs_AdvHealthcareMat.pdf | Main article | 4.49 MB | Adobe PDF | Ver/Abrir |
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