TY: JOUR T1 - Hydroperoxide production from linoleic acid by heterologous Gaeumannomyces graminis tritici lipoxygenase: Optimization and scale-up A1 - Villaverde, Juan Jose A1 - van der Vlist, Vincent A1 - Santos, Sonia A. O. A1 - Haarmann, Thomas A1 - Langfelder, Kim A1 - Pirttimaa, Minni A1 - Nyyssola, Antti A1 - Jylha, Sirpa A1 - Tamminen, Tarja A1 - Kruus, Kristiina A1 - de Graaff, Leo A1 - Neto, Carlos Pascoal A1 - Simoes, Mario M. Q. A1 - Domingues, M. R. M. A1 - Silvestre, Armando J. D. A1 - Eidner, Jasmin A1 - Buchert, Johanna N2 - Linoleic acid was converted into hydroperoxides by a Gaeumannomyces graminis tritici lipoxygenase produced recombinantly in Trichoderma reesei. Hydroperoxide production was optimized using a face-centred experimental design in order to study the effects of pH, temperature and time on the conversion of linoleic acid into four regioisomeric hydroperoxyoctadecadienoic acids (HPODE): 13-(Z,E)-, 9-(E,Z)-, 13-(E,E)-, 9-(E,E)-HPODE. Fitting equations described satisfactorily the system behavior and showed that reaction time was the most influencing independent variable. A set of independent variables (pH = 6.7, temperature = 23.9 degrees C and time = 18 h) allowed to obtain high yields of hydroperoxides (88.0%) with a good selectivity for the 13-(Z,E)-HPODE isomer (47.4%) when the initial substrate concentration was 10 g/L. The production was further investigated using industrially relevant linoleic acid concentrations (100-300 g/L) leading to HPODE yields of similar to 40% and the volumetric productivity 3.6 g/(L h), and a selectivity for 13-(Z,E)-HPODE of around 74%. (c) 2012 Elsevier B.V. All rights reserved. UR - https://ria.ua.pt/handle/10773/20786 Y1 - 2013 PB - ELSEVIER SCIENCE SA