Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/7732
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Saraiva, Jorge | pt |
dc.contributor.author | Oliveira, Jorge C. | pt |
dc.contributor.author | Lemos, Adília | pt |
dc.contributor.author | Hendrickx, Marc | pt |
dc.date.accessioned | 2012-04-04T11:04:47Z | - |
dc.date.issued | 1996 | - |
dc.identifier.issn | 1365-2621 | pt |
dc.identifier.uri | http://hdl.handle.net/10773/7732 | - |
dc.description.abstract | The thermal inactivation of horseradish peroxidase was studied in sodium phosphate buffer solutions and in pure water at pH 7 in the temperature range of 70–95°C. The sodium phosphate ions concentration affected both the thermostability and the kinetic patterns and had a stabilizing effect. The gradual change observed at low concentrations made a series-type mechanism theoretically more coherent with the experimental observations than the conventionally applied two-fraction model. In water the kinetics is apparently First order at high temperatures, while the results obtained at 25°C support the occurrence of a series-type inactivation mechanism. The pH and enzyme concentration also affect the inactivation proFile, supporting the conclusion that the thermal inactivation is not a monomolecular process with respect to protein concentration. | pt |
dc.language.iso | eng | pt |
dc.publisher | Wiley-Blackwell | pt |
dc.relation | AAIR programme - AIR1-CT92-0746 | pt |
dc.relation | JNICT | pt |
dc.rights | restrictedAccess | por |
dc.subject | Enzyme inactivation | pt |
dc.subject | Kinetic models | pt |
dc.title | Analysis of the kinetic patterns of horseradish peroxidase thermal inactivation in sodium phosphate buffer solutions of different ionic strength | pt |
dc.type | article | pt |
dc.peerreviewed | yes | pt |
ua.distribution | international | pt |
degois.publication.firstPage | 223 | pt |
degois.publication.issue | 3 | pt |
degois.publication.issue | 3 | - |
degois.publication.lastPage | 231 | pt |
degois.publication.title | International Journal of Food Science and Technology | pt |
degois.publication.volume | 31 | pt |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.doi | 10.1046/j.1365-2621.1996.00342.x | pt |
dc.identifier.doi | 10.1046/j.1365-2621.1996.00342.x | pt |
Appears in Collections: | QOPNA - Artigos |
Files in This Item:
File | Description | Size | Format | |
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P3_Peroxidase_IJFST Peroxidase kinetic patterns.pdf | 727.07 kB | Adobe PDF |
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