Utilize este identificador para referenciar este registo:
http://hdl.handle.net/10773/7732
Título: | Analysis of the kinetic patterns of horseradish peroxidase thermal inactivation in sodium phosphate buffer solutions of different ionic strength |
Autor: | Saraiva, Jorge Oliveira, Jorge C. Lemos, Adília Hendrickx, Marc |
Palavras-chave: | Enzyme inactivation Kinetic models |
Data: | 1996 |
Editora: | Wiley-Blackwell |
Resumo: | The thermal inactivation of horseradish peroxidase was studied in sodium phosphate buffer solutions and in pure water at pH 7 in the temperature range of 70–95°C. The sodium phosphate ions concentration affected both the thermostability and the kinetic patterns and had a stabilizing effect. The gradual change observed at low concentrations made a series-type mechanism theoretically more coherent with the experimental observations than the conventionally applied two-fraction model. In water the kinetics is apparently First order at high temperatures, while the results obtained at 25°C support the occurrence of a series-type inactivation mechanism. The pH and enzyme concentration also affect the inactivation proFile, supporting the conclusion that the thermal inactivation is not a monomolecular process with respect to protein concentration. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/7732 |
DOI: | 10.1046/j.1365-2621.1996.00342.x 10.1046/j.1365-2621.1996.00342.x |
ISSN: | 1365-2621 |
Aparece nas coleções: | QOPNA - Artigos |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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P3_Peroxidase_IJFST Peroxidase kinetic patterns.pdf | 727.07 kB | Adobe PDF |
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