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Title: Characterization of Phenolic Components in Polar Extracts of Eucalyptus globulus Labill. Bark by High-Performance Liquid Chromatography-Mass Spectrometry
Author: Santos, Sónia A. O.
Freire, Carmen S. R.
Domingues, M. Rosário M.
Silvestre, Armando J. D.
Neto, Carlos Pascoal
Keywords: Eucalyptus globulus Labill.
Phenolic compounds
Tandem mass spectrometry
Issue Date: 2011
Publisher: American Chemical Society
Abstract: High-performance liquid chromatography electrospray ionization mass spectrometry (HPLC-ESI-MS) and tandem mass spectrometry (MS") were used to investigate the phenolic constituents in methanol, water, and methanol/water extracts of Eucalyptus globulus Labill. bark. Twenty-nine phenolic compounds were identified, 16 of them referenced for the first time as constituents of E. globulus bark, namely, quinic, dihydroxyphenylacetic, and caffeic acids, bis(hexahydroxydiphenoyl (HHDP))-glucose, galloyl-bis(HHDP)-glucose, galloyl-HHDP-glucose, isorhamentin-hexoside, quercetin-hexoside, methylellagic acid (EA) pentose conjugate, myricetin-rhamnoside, isorhamnetin-rhamnoside, mearnsetin, phloridzin, mearnsetin-hexoside, luteolin, and a proanthocyanidin B-type dimer. Digalloylglucose was identified as the major compound in the methanol and methanol/water extracts, followed by isorhamnetin-rhamnoside in the methanol extract and by catechin in the methanol/water extract, whereas in the water extract catechin and galloyl- HHDP-glucose were identified as the predominant components. The methanol/water extract was shown be the most efficient to isolate phenolic compounds identified in E. globulus bark.
Peer review: yes
DOI: 10.1021/jf201801q
ISSN: 0021-8561
Appears in Collections:DQ - Artigos

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