Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/43194
Title: The influence of temperature on the impacts of caffeine in mussels: evaluating subcellular impacts and model predictions
Author: Cunha, Marta
Petrillo, Vincenzo
Madeira, Madalena
He, Yide
Coppola, Francesca
Meucci, Valentina
De Marchi, Lucia
Soares, Amadeu M. V. M.
Freitas, Rosa
Keywords: Marine bivalves
Climate change
Biomarkers
Stimulant drug
Integrated biomarker response
Independent action model
Issue Date: 25-Aug-2024
Publisher: Elsevier
Abstract: In aquatic ecosystems, the presence of pharmaceuticals, particularly caffeine (CAF), has been linked to wastewater discharge, hospital waste, and the disposal of expired pharmaceutical products containing CAF. Additionally, rising temperatures due to climate change are anticipated in aquatic environments. This study aimed to assess the toxicity of various CAF concentrations under current (17 °C) and projected (21 °C) temperature conditions, using the mussel Mytilus galloprovincialis as a bioindicator species. Subcellular impacts were evaluated following 28 days of exposure to four CAF concentrations (0.5; 1.0; 5.0; 10.0 μg/L) at the control temperature (17 °C). Only effects at an environmentally relevant CAF concentration (5.0 μg/L) were assessed at the highest temperature (21 °C). The overall biochemical response of mussels was evaluated using non-metric Multidimensional Scaling (MDS) and the Integrated Biomarker Response (IBR) index, while the Independent Action (IA) model was used to compare observed and predicted responses. Results showed that at 17 °C, increased CAF concentrations were associated with higher metabolism and biotransformation capacity, accompanied by cellular damage at the highest concentration. Conversely, under warming conditions (21 °C), the induction of antioxidant enzymes was observed, although insufficient to prevent cellular damage compared to the control temperature. Regarding neurotoxicity, at 17 °C, the activity of the acetylcholinesterase enzyme was inhibited up to 5.0 μg/L; however, at 10.0 μg/L, activity increased, possibly due to CAF competition for adenosine receptors. The IA model identified a synergistic response for most parameters when CAF and warming acted together, aligning with observed results, albeit with slightly lower magnitudes.
Peer review: yes
URI: http://hdl.handle.net/10773/43194
DOI: 10.1016/j.scitotenv.2024.173453
ISSN: 0048-9697
Appears in Collections:CESAM - Artigos

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