Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/42463
Full metadata record
DC FieldValueLanguage
dc.contributor.authorNunes, João C. F.pt_PT
dc.contributor.authorAlmeida, Mafalda R.pt_PT
dc.contributor.authorPaiva, Gabriela B. dept_PT
dc.contributor.authorPedrolli, Danielle B.pt_PT
dc.contributor.authorSantos-Ebinuma, Valéria C.pt_PT
dc.contributor.authorNeves, Márcia C.pt_PT
dc.contributor.authorFreire, Mara G.pt_PT
dc.contributor.authorTavares, Ana P. M.pt_PT
dc.date.accessioned2024-09-19T09:47:43Z-
dc.date.available2024-09-19T09:47:43Z-
dc.date.issued2023-07-
dc.identifier.isbn978-972-789-892-3-
dc.identifier.urihttp://hdl.handle.net/10773/42463-
dc.description.abstractL-asparaginase is an enzyme applied as a biopharmaceutical, as an acrylamide reduction agent and in the development of biosensors for the detection of L-asparagine (Nunes et al., 2020). L-asparaginase is widely distributed in nature, but microorganisms are the preferential source of production since they easily grow on inexpensive substrates (Lopes et al., 2017). For all the described applications, high levels of enzyme purity are required, reinforcing the need of developing cost-effective processes for the L-asparaginase purification. The major focus of this work is to develop an alternative and simple semi-continuous flow-through downstream process using Silica-based supported ionic liquid-like phase (SSILLP) materials for the purification of L-asparaginase. SSILLP materials comprise ionic liquids (ILs) covalently attached to silica, allowing distinct interactions to be established between the target compounds and the support. SSILLP materials based on quaternary ammonium cations with different alkyl chain lengths and the Cl anion were synthesized and characterized by elemental analysis, point of zero charge, Attenuated total reflectance-Fourier-transform infrared spectroscopy, Brunauer-Emmett-Teller surface area analysis, Barrett-Joyner-Halenda pore analysis, and scanning electron microscopy (SEM). An initial screening was performed to select the most promising SSILLP material for L-asparaginase purification. Optimization of L-asparaginase purification conditions, namely pH and solid/liquid ratio, was then carried out by Response Surface Methodology. Semi-continuous L-asparaginase purification was finally addressed using the best identified SSILLP material under optimized conditions (pH 3 and solid/liquid ratio of 15), attaining a purification factor of 5.15. Through this method, process costs could be significantly reduced, leading to the decrease of the enzyme price and thereby enabling its widespread application.pt_PT
dc.language.isoengpt_PT
dc.publisherUA Editorapt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50011%2F2020/PTpt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50011%2F2020/PTpt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0006%2F2020/PTpt_PT
dc.relationPOCI-01-0145-FEDER-031268pt_PT
dc.relation2018/06908–8pt_PT
dc.relation2019/15493–9pt_PT
dc.relationCEECIND/2020/01867pt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F00383%2F2017%2FCP1459%2FCT0031/PTpt_PT
dc.relation2021/01284-9pt_PT
dc.relation312463/2021-9pt_PT
dc.relation310023/2020-3pt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F150671%2F2020/PTpt_PT
dc.rightsopenAccesspt_PT
dc.subjectL-asparaginasept_PT
dc.subjectDownstream processingpt_PT
dc.subjectSupported ionic liquidspt_PT
dc.titleSupported ionic liquids for L-asparaginase downstream processingpt_PT
dc.typeconferenceObjectpt_PT
dc.description.versionpublishedpt_PT
dc.peerreviewedyespt_PT
ua.event.date12-14 July, 2023pt_PT
degois.publication.firstPage221pt_PT
degois.publication.lastPage222pt_PT
degois.publication.locationAveiropt_PT
degois.publication.titleResearch Summit 2023: book of abstractspt_PT
dc.relation.publisherversionhttps://ria.ua.pt/handle/10773/39666pt_PT
Appears in Collections:CICECO - Comunicações
DQ - Comunicações

Files in This Item:
File Description SizeFormat 
Research Summit 2023.pdf358.95 kBAdobe PDFView/Open


FacebookTwitterLinkedIn
Formato BibTex MendeleyEndnote Degois 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.