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Title: RUFY4 exists as two translationally regulated isoforms, that localize to the mitochondrion in activated macrophages
Author: Valečka, Jan
Camosseto, Voahirana
McEwan, David G.
Terawaki, Seigo
Liu, Zhuangzhuang
Strock, Eva
Almeida, Catarina R.
Su, Bing
Dikic, Ivan
Liang, Yinming
Gatti, Evelina
Pierre, Philippe
Keywords: RUFY
Alveolar macrophages
Issue Date: Jul-2021
Publisher: The Royal Society
Abstract: We report here that RUFY4, a newly characterized member of the 'RUN and FYVE domain-containing' family of proteins previously associated with autophagy enhancement, is highly expressed in alveolar macrophages (AM). We show that RUFY4 interacts with mitochondria upon stimulation by microbial-associated molecular patterns of AM and dendritic cells. RUFY4 interaction with mitochondria and other organelles is dependent on a previously uncharacterized OmpH domain located immediately upstream of its C-terminal FYVE domain. Further, we demonstrate that rufy4 messenger RNA can be translated from an alternative translation initiation codon, giving rise to a N-terminally truncated form of the molecule lacking most of its RUN domain and with enhanced potential for its interaction with mitochondria. Our observations point towards a role of RUFY4 in selective mitochondria clearance in activated phagocytes.
Peer review: yes
DOI: 10.1098/rsos.202333
Appears in Collections:IBIMED - Artigos

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