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Title: New method for isolating β-sitosterol from bleaching effluent of sulphite pulp mill
Author: Evtyugin, Dmitry D.
Prates, António
Domingues, M. Rosário
Casal, Susana
Evtuguin, Dmitry V.
Keywords: β-sitosterol
Sulphite pulping
Sulphite pulp bleaching
Organic solvent extraction
Eucalyptus wood
Issue Date: Aug-2023
Publisher: Elsevier
Abstract: β-sitosterol (BS) is a phytosterol with wide potential in cosmeceutical, biomedical and food applications. In acid sulphite pulp cooking, BS and its derivatives are considered an underutilized natural source of bioactive compounds, which are adsorbed on the resulting pulp or dispersed in the spent liquor. In this work, a new method for the isolation of BS from alkaline extract (AE) of unbleached sulphite pulp purification stage is proposed. BS was recovered by two-step acidification of AE up to pH 3 under pre-selected conditions, followed by fractionation of the formed precipitate with water-miscible organic solvents, having obtained best results (>90% of BS purity; yield of 70-210 mg/dm3 of AE) with methanol fractionation followed by BS crystallization induced via addition of water (ca. 10% vol.). When ethanol was used instead, BS was detected in low amounts in the isolate, being fatty acid sterol esters and fatty acid glycerides the major components, as revealed from analyses by wet chemistry coupled with gas chromatography-mass spectrometry and thin layer chromatography (TLC). The method is adaptable for large-scale industrial production of BS and may represent an important source of sterol-based products.
Peer review: yes
DOI: 10.1016/j.fbp.2023.05.007
ISSN: 0960-3085
Appears in Collections:CESAM - Artigos
CICECO - Artigos
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