Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/37716
Title: | Recovery of lactose and proteins from cheese whey with poly(ethylene)glycol/sulfate aqueous two-phase systems |
Author: | González Amado, Marlen Tavares, Ana P.M. Freire, Mara G. Soto, Ana Rodríguez, Oscar |
Keywords: | Aqueous two-phase systems Cheese whey Bovine serum albumin β-lactoglobulin α-lactalbumin Separation |
Issue Date: | 15-Jan-2021 |
Publisher: | Elsevier |
Abstract: | Cheese whey is an environmental problem as an effluent, but also a source of valuable raw materials, namely proteins and lactose. In this work, Aqueous Two-Phase Systems (ATPS) were studied for the recovery of lactose, BSA, β-lactoglobulin and α-lactalbumin, key components of cheese whey. ATPS formed by PEG (molecular weights: 200–8000 g.mol−1) with sodium or ammonium sulfate were investigated. Partitioning of the selected solutes was experimentally addressed in different ATPS and pH values. Partition behavior showed that ATPS formed by PEG1500/ammonium sulfate is able to separate lactose from proteins, while PEG300/sodium sulfate ATPS may be used for protein fractionation. These separation strategies were then tested with simulated and real cheese whey. Under optimized conditions, PEG 1500/ammonium sulfate ATPS allows efficient recovery of > 95% proteins (precipitate) and 80% of lactose (bottom phase), as confirmed for both simulated and real cheese whey. The results found indicate that the proposed polymer/salt ATPS can be used to design scalable and cost-effective separation strategies to apply in cheese whey and other related wastes. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/37716 |
DOI: | 10.1016/j.seppur.2020.117686 |
ISSN: | 1383-5866 |
Appears in Collections: | CICECO - Artigos DQ - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Recovery of lactose and proteins from Cheese Whey.pdf | 525.96 kB | Adobe PDF | View/Open |
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