Please use this identifier to cite or link to this item:
Title: Monitoring plasma protein aggregation during aging using conformation-specific antibodies and FTIR spectroscopy
Author: Magalhães, Sandra
Trindade, Dário
Martins, Tânia
Martins Rosa, Ilka
Delgadillo, Ivonne
Goodfellow, Brian J.
da Cruz E Silva, Odete A. B.
Henriques, Ana Gabriela
Nunes, Alexandra
Keywords: Protein aggregation
Conformation specific antibodies
Issue Date: Mar-2020
Publisher: Elsevier
Abstract: The loss of proteostasis during aging has been well described using different models, however little is known with respect to protein aggregation levels in biofluids with aging. Therefore, the aim of this study was to assess the pattern of age-related protein aggregation in human plasma using two distinct approaches: analysis with conformation-specific antibodies and FTIR spectroscopy. The latter has been widely used in biomedical research to study protein conformational changes in health and disease. Samples from a primary care based-cohort from the Aveiro region, Portugal, were used for slot-blot analyses followed by immunodetection with conformation-specific antibodies and for the acquisition of FTIR spectra. Immunoblot analyses revealed an age-dependent evolution of the protein conformational profile in human plasma, towards a decrease in prefibrillar oligomers and an increase in fibrillar structures. This finding was also supported by PLS-R multivariate analysis of FTIR data, where a positive correlation between the age of the donors and secondary structure of plasma proteins could be observed. Samples from younger donors are characterized by antiparallel β-sheet-containing structures while intermolecular β-sheets characterized older samples. Exclusion of age-associated co-morbidities improved the correlation between protein conformational profiles and aging. The results reveal structural changes in human plasma proteins from middle to old age, confirming the age-associated changes in protein aggregation, and support the applicability of FTIR as a reliable approach to study proteostasis during aging.
Peer review: yes
DOI: 10.1016/j.cca.2019.11.025
ISSN: 0009-8981
Appears in Collections:CICECO - Artigos
IBIMED - Artigos
DQ - Artigos
DCM - Artigos

Files in This Item:
File Description SizeFormat 
Monitoring plasma protein aggregation during aging using conformation-specific.pdf1.04 MBAdobe PDFView/Open

Formato BibTex MendeleyEndnote Degois 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.