Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/37594
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dc.contributor.authorFerreira, Nuno G. C.pt_PT
dc.contributor.authorMorgado, Rui G.pt_PT
dc.contributor.authorCunha, Luíspt_PT
dc.contributor.authorNovo, Martapt_PT
dc.contributor.authorSoares, Amadeu M. V. M.pt_PT
dc.contributor.authorMorgan, Andrew J.pt_PT
dc.contributor.authorLoureiro, Susanapt_PT
dc.contributor.authorKille, Peterpt_PT
dc.date.accessioned2023-05-08T13:54:43Z-
dc.date.available2023-05-08T13:54:43Z-
dc.date.issued2019-09-
dc.identifier.issn0013-9351pt_PT
dc.identifier.urihttp://hdl.handle.net/10773/37594-
dc.description.abstractDuring the last few years, there has been an alarming increase in the amount of nickel (Ni) being released into the environment, primarily due to its use in the production of stainless steel but also from other sources such as batteries manufacturing and consequent disposal. The established biotic ligand models provide precise estimates for Ni bioavailability, in contrast, studies describing the mechanisms underpinning toxicological effect of Ni are scarce. This study exploits RNA-seq to determine the transcriptomic responses of isopods using Porcellionides pruinosus as an example of a terrestrial metal-resistant woodlouse. Furthermore, the recently proposed model for Ni adverse outcome pathways (Ni-AOP) presents an unprecedented opportunity to fit isopod responses to Ni toxicity and define Porcellionides pruinosus as a metalomic model. Prior to this study, P. pruinosus represented an important environmental sentinel, though lacking genetic/omic data. The reference transcriptome generated here thus represents a major advance and a novel resource. A detailed annotation of the transcripts obtained is presented together with the homology to genes/gene products from Metazoan and Arthropoda phylum, Gene Ontology (GO) classification, clusters of orthologous groups (COG) and assignment to KEGG metabolic pathways. The differential gene expression comparison was determined in response to nickel (Ni) exposure and used to derive the enriched pathways and processes. It revealed a significant impact on ion trafficking and storage, oxidative stress, neurotoxicity, reproduction impairment, genetics and epigenetics. Many of the processes observed support the current Ni-AOP although the data highlights that the current model can be improved by including epigenetic endpoints, which represents key chronic risks under a scenario of Ni toxicity.pt_PT
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relationFCOMP-01-0124-FEDER-008656pt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FAMB%2F50017%2F2019/PTpt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F65739%2F2009/PTpt_PT
dc.relationH2020-COFUND-SIRCIW>MINT-512202pt_PT
dc.relationMSCA–IF–2014-GF-660378pt_PT
dc.relationBPD/UI88/6010/2016pt_PT
dc.rightsopenAccesspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectTranscriptomept_PT
dc.subjectRNA-Seq analysispt_PT
dc.subjectTerrestrial isopodspt_PT
dc.subjectMetal traffickingpt_PT
dc.titleUnravelling the molecular mechanisms of nickel in woodlicept_PT
dc.typearticlept_PT
dc.description.versionpublishedpt_PT
dc.peerreviewedyespt_PT
degois.publication.titleEnvironmental researchpt_PT
degois.publication.volume176pt_PT
dc.identifier.doi10.1016/j.envres.2019.05.038pt_PT
dc.identifier.articlenumber108507pt_PT
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DBio - Artigos

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