Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/37081
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dc.contributor.authorPereira, Isabelpt_PT
dc.contributor.authorSimões, Joanapt_PT
dc.contributor.authorEvtyugin, Dmitry V.pt_PT
dc.contributor.authorRouif, Sophiept_PT
dc.contributor.authorCoimbra, Manuel A.pt_PT
dc.contributor.authorDomingues, M. Rosário M.pt_PT
dc.contributor.authorGama, Miguelpt_PT
dc.date.accessioned2023-04-17T08:23:51Z-
dc.date.available2023-04-17T08:23:51Z-
dc.date.issued2018-06-
dc.identifier.issn0014-3057pt_PT
dc.identifier.urihttp://hdl.handle.net/10773/37081-
dc.description.abstractSterilization of biomaterials by gamma irradiation should not change its structure, since this may affect also its bioactivity. In this work, dextrin and periodate-oxidized dextrin (ODEX), both irradiated and non-irradiated, were hydrolysed by α-amylase and the obtained oligosaccharides were fractionated by ligand-exchange/size exclusion chromatography and analyzed by matrix-assisted laser desorption/ionization (MALDI-MS) and electrospray ionization (ESI-MSn) mass spectrometry. This allowed the identification of structures formed during partial periodate oxidation, which displayed aldehyde groups in different positions of dextrin backbone, according to the type of residue which was oxidized. Importantly, gamma irradiation did not structurally change either dextrin or ODEX, showing that it can be used as suitable terminal sterilization method of these materials for biomedical applications. To our best knowledge, this is the first report using MS-based techniques to evaluate the effects of partial periodate oxidation and of gamma irradiation on polysaccharides and, in particular, on dextrin and its oxidized form.pt_PT
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F90066%2F2012/PTpt_PT
dc.relationNorte-07-0202-FEDER-038853pt_PT
dc.relationNORTE-01-0145-FEDER-000004pt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FQUI%2F00062%2F2013/PTpt_PT
dc.relationREDE/1504/REM/2005pt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F90447%2F2012/PTpt_PT
dc.rightsopenAccesspt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectDextrinpt_PT
dc.subjectPeriodate oxidationpt_PT
dc.subjectGamma irradiationpt_PT
dc.subjectElectrospray ionization mass spectrometrypt_PT
dc.subjectMALDI-MSpt_PT
dc.subjectBiomedical applicationpt_PT
dc.titleEffects of gamma irradiation and periodate oxidation on the structure of dextrin assessed by mass spectrometrypt_PT
dc.typearticlept_PT
dc.description.versionpublishedpt_PT
dc.peerreviewedyespt_PT
degois.publication.firstPage158pt_PT
degois.publication.lastPage169pt_PT
degois.publication.titleEuropean Polymer Journalpt_PT
degois.publication.volume103pt_PT
dc.identifier.doi10.1016/j.eurpolymj.2018.04.011pt_PT
dc.identifier.essn1873-1945pt_PT
Appears in Collections:CICECO - Artigos
DQ - Artigos
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