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Title: Discovery of bioactive nitrated lipids and nitro-lipid-protein adducts using mass spectrometry-based approaches
Author: Melo, Tânia
Montero-Bullón, Javier-Fernando
Domingues, Pedro
Domingues, M. Rosário
Issue Date: May-2019
Publisher: Elsevier
Abstract: Nitro-fatty acids (NO2-FA) undergo reversible Michael adduction reactions with cysteine and histidine residues leading to the post-translational modification (PTM) of proteins. This electrophilic character of NO2-FA is strictly related to their biological roles. The NO2-FA-induced PTM of signaling proteins can lead to modifications in protein structure, function, and subcellular localization. The nitro lipid-protein adducts trigger a series of downstream signaling events that culminates with anti-inflammatory, anti-hypertensive, and cytoprotective effects mediated by NO2-FA. These lipoxidation adducts have been detected and characterized both in model systems and in biological samples by using mass spectrometry (MS)-based approaches. These MS approaches allow to unequivocally identify the adduct together with the targeted residue of modification. The identification of the modified proteins allows inferring on the possible impact of the NO2-FA-induced modification. This review will focus on MS-based approaches as valuable tools to identify NO2-FA-protein adducts and to unveil the biological effect of this lipoxidation adducts.
Peer review: yes
DOI: 10.1016/j.redox.2019.101106
Appears in Collections:CESAM - Artigos
DQ - Artigos
QOPNA - Artigos

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