Please use this identifier to cite or link to this item:
Title: A multi-endpoint approach to the combined toxic effects of patulin and ochratoxin a in human intestinal cells
Author: Assunção, Ricardo
Pinhão, Mariana
Loureiro, Susana
Alvito, Paula
Silva, Maria João
Keywords: Mycotoxin mixtures
Gastrointestinal toxicity
Interaction effects
Barrier integrity
Issue Date: 1-Oct-2019
Publisher: Elsevier
Abstract: Humans can be exposed to a complex and variable combination of mycotoxins. After ingestion, intestinal mucosa constitutes the first biological barrier that can be exposed to high concentrations of these toxins. The present study aimed to characterize the combined cytotoxicity, genotoxicity and impact on the gastrointestinal barrier integrity of patulin (PAT, 0.7 μM to 100 μM) and ochratoxin A (OTA, 1 μM to 200 μM) mixtures in Caco-2 cells. A dose-ratio deviation was verified for cytotoxicity, implying that OTA was mainly responsible for synergism when dominant in the mixture, while this pattern was changed to antagonism for the highest PAT concentrations. Genotoxicity (comet assay) results were compatible with an interactive DNA damaging effect at the highest PAT and OTA concentrations, not clearly mediated by the formation of oxidative DNA breaks. Regarding gastrointestinal barrier integrity, a potential synergism was attained at low levels of both mycotoxins, changing to antagonism at higher doses. The present results indicate that combined mycotoxins effects may arise at the intestinal level and should not be underestimated when evaluating their risk to human health.
Peer review: yes
DOI: 10.1016/j.toxlet.2019.06.002
ISSN: 0378-4274
Appears in Collections:CESAM - Artigos
DBio - Artigos

Files in This Item:
File Description SizeFormat 
A multi-endpoint approach to the combined toxic effects.pdf799.43 kBAdobe PDFView/Open

Formato BibTex MendeleyEndnote Degois 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.