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Title: Isolation and identification of an arabinogalactan extracted from pistachio external hull: assessment of immunostimulatory activity
Author: Hamed, Mariem
Coelho, Elisabete
Bastos, Rita
Evtuguin, Dmitry V
Ferreira, Sónia S
Lima, Tânia
Vilanova, Manuel
Sila, Assaad
Coimbra, Manuel A
Bougatef, Ali
Keywords: Arabinogalactan-protein
Structural characterization
Methylation analysis
NMR spectroscopy
Immunostimulatory activity
Issue Date: 30-Mar-2022
Publisher: Elsevier
Abstract: This work studies the extraction and purification of a novel arabinogalactan from pistachio external hull. It was extracted with a simple method from pistachio hull which is considered as unexploited waste. Based on the results of sugar analysis by GC-FID, glycosidic linkage by GC-MS, NMR spectroscopy, and molecular weight by Size Exclusion Chromatography, pistachio hull water soluble polysaccharides (PHWSP) were identified as a type II arabinogalactan (AG), with characteristic terminally linked α-Araf, (α1 → 5)-Araf, (α1 → 3,5)-Araf, terminally linked β-Galp, (β1 → 6)-Galp, and (β1 → 3,6)-Galp. DEPT-135, HSQC, HMBC and COSY NMR data suggested the presence of (β1 → 3)-Galp mainly branched at O-6 with (β1 → 6)-Galp chains, α-Araf chains, and terminally linked α-Araf. These AG from pistachio external hulls showed in vitro stimulatory activity for B cells, suggesting their possible use as an immunological stimulant in nutraceutical and biomedical applications.
Peer review: yes
DOI: 10.1016/j.foodchem.2021.131416
ISSN: 0308-8146
Appears in Collections:CICECO - Artigos

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