Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/35576
Full metadata record
DC FieldValueLanguage
dc.contributor.authorMartins, Margaridapt_PT
dc.contributor.authorOoi, Chien Weipt_PT
dc.contributor.authorNeves, Márcia C.pt_PT
dc.contributor.authorPereira, Jorge F. B.pt_PT
dc.contributor.authorCoutinho, João A. P.pt_PT
dc.contributor.authorVentura, Sónia P. M.pt_PT
dc.date.accessioned2023-01-03T14:34:25Z-
dc.date.available2023-01-03T14:34:25Z-
dc.date.issued2018-07-
dc.identifier.issn0268-2575pt_PT
dc.identifier.urihttp://hdl.handle.net/10773/35576-
dc.description.abstractGreen fluorescent protein (GFP) is used extensively as a biomarker due to its unique spectral and fluorescence characteristics. GFP is usually obtained from recombinant strains of Escherichia coli (E. coli) producing the protein intracellularly. However, the common methods of extraction are cumbersome leading to an increase in the downstream process costs and complexity, sometimes leading to a higher risk of biomolecule degradation. RESULTS: This work proposes a new method to extract recombinant intracellular GFP from E. coli BL21 by using aqueous solutions of surface-active compounds. CONCLUSION: By comparing the fluorescence intensity of the extracted GFP, it was concluded that some of these compounds, namely ionic liquids with an alkyl chain of 10 or more carbons (best solvent being tributyl-1-tetradecylphosphonium, [P4,4,4,14]Cl) are more effective than an ultrasonic-assisted extraction, even at low concentrations, being able to extract the whole GFP content from the cellspt_PT
dc.language.isoengpt_PT
dc.publisherWiley-Blackwellpt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FCTM%2F50011%2F2013/PTpt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F110423%2F2015/PTpt_PT
dc.relationinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F122220%2F2016/PTpt_PT
dc.rightsrestrictedAccesspt_PT
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCell disruptionpt_PT
dc.subjectExtractionpt_PT
dc.subjectGreen fluorescent proteinpt_PT
dc.subjectIntracellular proteinpt_PT
dc.subjectRecombinant proteinspt_PT
dc.subjectSurface-active compoundspt_PT
dc.titleExtraction of recombinant proteins from Escherichia coli by cell disruption with aqueous solutions of surface-active compoundspt_PT
dc.typearticlept_PT
dc.description.versionpublishedpt_PT
dc.peerreviewedyespt_PT
degois.publication.firstPage1864pt_PT
degois.publication.issue7pt_PT
degois.publication.lastPage1870pt_PT
degois.publication.titleJournal of Chemical Technology & Biotechnologypt_PT
degois.publication.volume93pt_PT
dc.identifier.doi10.1002/jctb.5596pt_PT
dc.identifier.essn1097-4660pt_PT
Appears in Collections:CICECO - Artigos
DQ - Artigos

Files in This Item:
File Description SizeFormat 
Z.pdf849.15 kBAdobe PDFrestrictedAccess


FacebookTwitterLinkedIn
Formato BibTex MendeleyEndnote Degois 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.