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Title: Synthesis and characterization of surface-active ionic liquids used in the disruption of escherichia coli cells
Author: Sintra, Tânia E
Vilas, Miguel
Martins, Margarida
Ventura, Sónia P. M.
Lobo Ferreira, Ana I. M. C.
Santos, Luís M. N. B. F.
Gonçalves, Fernando J. M.
Tojo, Emília
Coutinho, João A. P.
Keywords: Ammonium Compounds
Escherichia coli
Green Fluorescent Proteins
Ionic Liquids
Organophosphorus Compounds
Surface Properties
Issue Date: 4-Mar-2019
Publisher: Wiley-Blackwell
Abstract: Twelve surface-active ionic liquids (SAILs) and surface-active derivatives, based on imidazolium, ammonium, and phosphonium cations and containing one, or more, long alkyl chains in the cation and/or the anion, were synthetized and characterized. The aggregation behavior of these SAILs in water, as well as their adsorption at solution/air interface, were studied by assessing surface tension and conductivity. The CMC values obtained (0.03-6.0 mM) show a high propensity of these compounds to self-aggregate in aqueous media. Their thermal properties were also characterized, namely the melting point and decomposition temperature by using DSC and TGA, respectively. Furthermore, the toxicity of these SAILs was evaluated using the marine bacteria Aliivibrio fischeri (Gram-negative). According to the EC50 values obtained (0.3-2.7 mg L-1 ), the surface-active compounds tested should be considered "toxic" or "highly toxic". Their ability to induce cell disruption of Escherichia coli cells (also Gram-negative), releasing the intracellular green fluorescent protein (GFP) produced, was investigated. The results clearly evidence the capability of these SAILs to act as cell disruption agents.
Peer review: yes
DOI: 10.1002/cphc.201801127
ISSN: 1439-4235
Appears in Collections:CESAM - Artigos
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