Please use this identifier to cite or link to this item:
Title: Sequential recovery of C-phycocyanin and chlorophylls from Anabaena cylindrica
Author: Sintra, Tânia E.
Bagagem, Sara S.
Ghazizadeh Ahsaie, Farzaneh
Fernandes, Andreia
Martins, Margarida
Macário, Inês P.E.
Pereira, Joana L.
Gonçalves, Fernando J.M.
Pazuki, Gholamreza
Coutinho, João A.P.
Ventura, Sónia P.M.
Keywords: Cyanobacteria
Anabaena cylindrica
Sequential extraction
Aqueous biphasic systems
Issue Date: 2021
Publisher: Elsevier
Abstract: Cyanobacteria are attracting the attention worldwide as a reliable and sustainable feedstock for the production of biofuels, food colorants and biochemically active compounds. Anabaena cylindrica (A. cylindrica) is a cya- nobacterium with a significant widespread occurrence in Portuguese freshwaters, with C-phycocyanin and chlorophylls, compounds with a high market value, being abundantly produced by the species. This work presents the development of a sequential downstream process to obtain C-phycocyanin and chlorophyll from A. cylindrica. The C-phycocyanin extraction is carried using Na-phosphate (20 mM, pH 7) buffer, while aqueous solutions of surface-active compounds (250 mM), as well as ethanol, were screened for the extraction of the chlorophylls. After selecting the best solvents to recover both pigments from A. cylindrica, the operational conditions (solid-liquid ratio, time of extraction and temperature) were optimized, allowing the recovery of around 90% and 55% of the total content of C-phycocyanin and chlorophylls, respectively. Finally, dextran/ copolymer-based ABS were applied to promote the purification of C-phycocyanin from the residual chlorophylls and other contaminant proteins, leading to an increase in the C-phycocyanin purity of 4-fold, without com- promising its photostability.
Peer review: yes
DOI: 10.1016/j.seppur.2020.117538
ISSN: 1383-5866
Appears in Collections:CICECO - Artigos

Files in This Item:
File Description SizeFormat 
F.pdf458.12 kBAdobe PDFrestrictedAccess
1-s2.0-S1383586620320128-main.pdf4.31 MBAdobe PDFrestrictedAccess

Formato BibTex MendeleyEndnote Degois 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.