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Title: Purification of immunoglobulin Y from egg yolk using thermoresponsive aqueous micellar two-phase systems comprising ionic liquids
Author: Vicente, Filipa A.
Castro, Leonor S.
Mondal, Dibyendu
Coutinho, João A. P.
Tavares, Ana P. M.
Ventura, Sónia P. M.
Freire, Mara G.
Keywords: Immunoglobulin Y
Thermoresponsive system
Micellar systems
Surface-active ionic liquids
Issue Date: 1-May-2022
Publisher: Elsevier
Abstract: Immunoglobulin Y (IgY) represents an important class of antibodies, being present in egg yolk, with relevant medical applications and involving non-invasive methods of extraction. However, due to the complexity of egg yolk, the purification levels required for use in most medical applications demands the application of multi-step and cost-intensive techniques. Therefore, it is of upmost importance to develop a biocompatible and cost-effective downstream process to purify IgY from egg yolk. In this work, IgY was purified from the egg yolk water-soluble protein fraction (WSPF) by the application of thermoresponsive aqueous micellar two-phase systems (AMTPS) composed of the non-ionic surfactant Triton X-114 and surface-active ionic liquids (SAILs) as co-surfactants. The best thermoresponsive systems allowed, in a single step, to recover IgY in the surfactant-poor phase with a purity level of 69%, and up to 73% if consecutive extraction cycles are performed, while maintaining the structural integrity of the antibodies. Considering these results, a downstream process was designed and proposed, consisting of four main steps: (i) recovery of the WSPF; (ii) purification of IgY by applying AMTPS; (iii) isolation of the IgY from the main solvents using an ultrafiltration step; (iv) recycling of the AMTPS phase-forming components by precipitating the contaminant proteins.
Peer review: yes
DOI: 10.1016/j.seppur.2022.120589
ISSN: 1383-5866
Appears in Collections:CICECO - Artigos
DQ - Artigos

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