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Title: Bioengineered hierarchical bonelike compartmentalized microconstructs using nanogrooved microdiscs
Author: Bjørge, Isabel M.
Sousa, Bárbara M. de
Patrício, Sónia G.
Silva, Ana Sofia
Nogueira, Liebert P.
Santos, Lúcia F.
Vieira, Sandra I.
Haugen, Håvard J.
Correia, Clara R.
Mano, João F
Keywords: Biophysical cues
Liquefied-core capsules
Nanogrooved microdiscs
Bottom-up tissue engineering
Cell self-assembly
Issue Date: 21-Apr-2022
Publisher: American Chemical Society
Abstract: Fabrication of vascularized large-scale constructs for regenerative medicine remains elusive since most strategies rely solely on cell self-organization or overly control cell positioning, failing to address nutrient diffusion limitations. We propose a modular and hierarchical tissue-engineering strategy to produce bonelike tissues carrying signals to promote prevascularization. In these 3D systems, disc-shaped microcarriers featuring nanogrooved topographical cues guide cell behavior by harnessing mechanotransduction mechanisms. A sequential seeding strategy of adipose-derived stromal cells and endothelial cells is implemented within compartmentalized, liquefied-core macrocapsules in a self-organizing and dynamic system. Importantly, our system autonomously promotes osteogenesis and construct's mineralization while promoting a favorable environment for prevascular-like endothelial organization. Given its modular and self-organizing nature, our strategy may be applied for the fabrication of larger constructs with a highly controlled starting point to be used for local regeneration upon implantation or as drug-screening platforms.
Peer review: yes
DOI: 10.1021/acsami.2c01161
ISSN: 1944-8244
Appears in Collections:CICECO - Artigos
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