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http://hdl.handle.net/10773/34578
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DC Field | Value | Language |
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dc.contributor.author | Santos, Lúcia F. | pt_PT |
dc.contributor.author | Silva, A. Sofia | pt_PT |
dc.contributor.author | Mano, João F. | pt_PT |
dc.date.accessioned | 2022-09-09T10:59:13Z | - |
dc.date.issued | 2020-12 | - |
dc.identifier.issn | 1742-7061 | pt_PT |
dc.identifier.uri | http://hdl.handle.net/10773/34578 | - |
dc.description.abstract | The use of cells as building blocks for tissue engineering purposes has been a matter of research in the recent years. Still, the fabrication of complex-shaped 3D-like constructs using living-based materials is hampered through the difficulty in recapitulating the mechanical properties of the native tissues. In an attempt to develop robust tissue-like constructs, it is herein proposed the fabrication of complex-shaped magnetic cell sheets (CSs) with improved mechanical properties for bone TE. Hence, magnetic CSs with versatile shapes and enhanced mechanical performance are fabricated using a pre-osteoblast cell line (MC3T3-E1) through an universal approach that relies on the design of the substrate, cell density and magnetic force. Results show that such magnetic CSs exhibit a Young's modulus similar to those encountered in the soft tissues. The construction of stratified CSs is also explored using MC3T3-E1 and adipose-derived stromal cells (ASCs). The role of the pre-osteoblast cell line on ASCs osteogenesis is herein investigated for the first time in layered scaffold-free structures. After 21 days, the level of osteogenic markers in the heterotypic CS (MC3T3-E1:ASCs) is significantly higher than in the homotypic one (ASCs:ASCs), even in the absence of osteogenic differentiation factors. These evidences open new prospects for the creation of mechanically robust, complex, higher-ordered and completely functional 3D cell-based materials that better resemble the native environment of in vivo tissues. | pt_PT |
dc.language.iso | eng | pt_PT |
dc.publisher | Elsevier | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50011%2F2020/PT | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBTM-MAT%2F29830%2F2017/PT | pt_PT |
dc.relation | ERC-2014-ADG-669858 | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50011%2F2020/PT | pt_PT |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F141523%2F2018/PT | pt_PT |
dc.rights | embargoedAccess | pt_PT |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | pt_PT |
dc.subject | Complex-shape | pt_PT |
dc.subject | Magnetic cell sheet | pt_PT |
dc.subject | Mechanical behavior | pt_PT |
dc.subject | Tissue engineering | pt_PT |
dc.subject | Robustness | pt_PT |
dc.title | Complex-shaped magnetic 3D cell-based structures for tissue engineering | pt_PT |
dc.type | article | pt_PT |
dc.description.version | published | pt_PT |
dc.peerreviewed | yes | pt_PT |
degois.publication.firstPage | 18 | pt_PT |
degois.publication.lastPage | 31 | pt_PT |
degois.publication.title | Acta Biomaterialia | pt_PT |
degois.publication.volume | 118 | pt_PT |
dc.date.embargo | 2023-01-01 | - |
dc.identifier.doi | 10.1016/j.actbio.2020.10.005 | pt_PT |
dc.identifier.essn | 1878-7568 | pt_PT |
Appears in Collections: | CICECO - Artigos DQ - Artigos |
Files in This Item:
File | Description | Size | Format | |
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Complex-shaped magnetic 3D cell-based structures for tissue engineering - Revised Manuscript.pdf | 2.9 MB | Adobe PDF | View/Open |
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