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Title: Saliva as a non-invasive specimen for COPD assessment
Author: Melo-Dias, Sara
Valente, Carla
Andrade, Lília
Marques, Alda
Sousa, Ana
Keywords: Microbiota
Salivary bacteria
Respiratory diseases
Issue Date: 29-Jan-2022
Publisher: BMC
Abstract: Background: People with COPD have been reported to bear a distinct airway microbiota from healthy individuals based on bronchoalveolar lavage (BAL) and sputum samples. Unfortunately, the collection of these samples involves relatively invasive procedures and is resource-demanding, limiting its regular use. Non-invasive samples from the upper airways could constitute an interesting alternative, but its relationship with COPD is still underexplored. We examined the merits of saliva to identify the typical profle of COPD oral bacteria and test its association with the disease. Methods: Outpatients with COPD and age-sex matched healthy controls were recruited and characterised based on clinical parameters and 16S rRNA profling of oral bacteria. A clustering analysis based on patients’ oral bacteria betadiversity and logistic regressions were performed to evaluate the association between oral bacteria composition and COPD. Results: 128 individuals participated (70 patients and 58 controls). Diferential abundance analyses showed difer‑ ences in patients comparable to the ones previously observed in samples from the lower respiratory tract, i.e., an increase in Proteobacteria (particularly Haemophilus) and loss of microbiota diversity. An unsupervised clustering analysis separated patients in two groups based on microbiota composition difering signifcantly in the frequency of patients hospitalized due to severe acute exacerbation of COPD (AECOPD) and in the frequency of GOLD D patients. Furthermore, a low frequency of Prevotella was associated with a signifcantly higher risk of recent severe AECOPD and of being GOLD D. Conclusion: Salivary bacteria showed an association with COPD, particularly with severe exacerbations, supporting the use of this non-invasive specimen for future studies of heterogeneous respiratory diseases like COPD.
Peer review: yes
DOI: 10.1186/s12931-022-01935-9
ISSN: 1465-9921
Appears in Collections:IBIMED - Artigos
ESSUA - Artigos
DCM - Artigos
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