Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorXinqing Pangpt_PT
dc.contributor.authorO'Malley, Clarept_PT
dc.contributor.authorBorges, Joãopt_PT
dc.contributor.authorRahman, Muhammad M.pt_PT
dc.contributor.authorCollis, Dominic W. P.pt_PT
dc.contributor.authorMano, João F.pt_PT
dc.contributor.authorMackenzie, Ian C.pt_PT
dc.contributor.authorAzevedo, Helena S.pt_PT
dc.description.abstractThe supramolecular presentation of extracellular matrix components on surfaces provides a platform for the investigation and control of cell behavior. Hyaluronan (HA) is one of the main components of the extracellular environment and has been shown to play an important role in different cancers and their progression. However, current methods of HA immobilization often require its chemical modification. Herein, a peptide-based self-assembled monolayer (SAM) is used as an anchor to immobilize unmodified HA on a bare gold surface, as demonstrated by the quartz crystal microbalance with dissipation monitoring. Peptide-HA surfaces show increased roughness and greater hydrophobicity when compared to poly-D-lysine/HA surfaces, as measured by atomic force microscopy and water contact angle, respectively. Additionally, the peptide SAM can be micro-contact printed and used to restrict the presentation of HA to specific regions, thereby creating HA patterned surfaces to examine cell behavior. When used for cell culture, these surfaces result in altered adhesion and migration of LUC4 head and neck squamous cell carcinoma cells. These biomimetic surfaces can provide insights into the role of HA in cancer and other diseases and be used as a platform for the development of cell sorting devices.pt_PT
dc.description.sponsorshipC.O’M. thanks The Queen Mary Institute of Bioengineering and the Engineering and Physical Sciences Research Council for financial support through a Ph.D. studentship (Award number 1502316). J.B. gratefully acknowledges the financial support by Fundação para a Ciência e a Tecnologia (FCT), I.P., through individual contract (CEECIND/03202/2017). This work was also funded by national funds (OE), through FCT, I.P., in the scope of the framework contract foreseen in the numbers 4, 5, and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19. The authors also acknowledge the financial support from the EU-funded project “SuprHApolymers” (PCIG14-GA-2013-631871).pt_PT
dc.subjectCancer stem cellspt_PT
dc.subjectModel surfacespt_PT
dc.subjectSelf-assembled monolayerspt_PT
dc.titleSupramolecular presentation of hyaluronan onto model surfaces for studying the behavior of cancer stem cellspt_PT
degois.publication.titleAdvanced Biosystemspt_PT
Appears in Collections:CICECO - Artigos
DQ - Artigos

Files in This Item:
File Description SizeFormat 
Advanced Biosystems 2019.pdf1.57 MBAdobe PDFrestrictedAccess

Formato BibTex MendeleyEndnote Degois 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.