Proteomic analysis of tissue-specific protein aggregation signatures throughout mammalian aging

Abstract

Proteome and proteostasis network ( disruptions lead to proteostasis imbalances and accumulation of protein aggregates, characteristic of several age related diseases 1 3 The consequences of PN disturbances in the context of healthy aging remains to be thoroughly studied in mammals To determine whether accumulation of insoluble proteins occurs during mammalian aging, young adult 6 month old), middle aged 13 month old), and old 18 and 24 month old) C 57 BL/ 6 mice were used to obtain detergent insoluble fractions Detergent insoluble and soluble fractions were isolated from total protein extracts of the liver, followed by characterization of total, detergent soluble, and detergent insoluble protein profiles We performed SWATH mass spectrometry analysis to identify differential proteome signatures of aging and determine which proteins are more prone to aggregate and further elucidated the altered functions and biological processes.