Please use this identifier to cite or link to this item: http://hdl.handle.net/10773/31485
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dc.contributor.authorPereira, Marisapt_PT
dc.contributor.authorRibeiro, Diana R.pt_PT
dc.contributor.authorPinheiro, Miguel M.pt_PT
dc.contributor.authorFerreira, Margaridapt_PT
dc.contributor.authorKellner, Stefaniept_PT
dc.contributor.authorSoares, Ana R.pt_PT
dc.date.accessioned2021-06-25T10:32:42Z-
dc.date.available2021-06-25T10:32:42Z-
dc.date.issued2021-
dc.identifier.urihttp://hdl.handle.net/10773/31485-
dc.description.abstractTransfer RNAs (tRNAs) are subjected to a wide variety of post-transcriptional modifications to ensure their structural stability, correct folding, and efficient protein decoding. More specifically, modifications in the D- and T-loops of tRNAs are essential for tRNA stability. However, the biological role of the tRNA epitranscriptome in the generation of tRNA-derived small RNA fragments (tsRNAs), a class of small non-coding RNAs, is still not totally understood. The 5-methyluridine (m5U) modification at position 54 of cytosolic tRNAs is one of the most common and conserved tRNA modifications among species. In mammals, this modification is catalyzed by the tRNA methyltransferase TRMT2A. To study the relevance of m5U54 for tRNA-derived small RNA formation, we have knockdown TRMT2A in human cells and found that m5U54 hypomodification resulted in ANG overexpression and tRNA cleavage near the anticodon, with accumulation of 5’tRNA-derived stress-induced RNAs (5’tiRNAs), in particular 5’tiRNA-GlyGCC and 5’tiRNA-GluCTC. Moreover, we found that exposure to oxidative stress induces TRMT2A downregulation, ANG overexpression and tsRNA generation. Our results establish a direct link between tRNA demethylation and ANG-dependent tRFs formation and propose the m5U54 as a tRNA cleavage protective mark.pt_PT
dc.language.isoengpt_PT
dc.relationSFRH/BD/135655/2018pt_PT
dc.relationSFRH/BD/146703/2019pt_PT
dc.relationPOCI-01-0145-FEDER-016630pt_PT
dc.relationPOCI-01-0145-FEDER-029843pt_PT
dc.relationCentro-01-0145-FEDER-000003pt_PT
dc.relationH2020-WIDESPREAD-2020-5 ID-952373pt_PT
dc.rightsopenAccesspt_PT
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjecttRNApt_PT
dc.subjectNon-coding RNAspt_PT
dc.subjectTranscriptomicspt_PT
dc.subjectEpitranscriptomept_PT
dc.titleLack of TRMT2A, a tRNA methyltransferase, leads to m5U54 tRNA hypomodification and generation of tRNA-derived small RNAspt_PT
dc.typeconferenceObjectpt_PT
dc.description.versionpublishedpt_PT
dc.peerreviewedyespt_PT
ua.event.date21-23 julho, 2021pt_PT
degois.publication.titlencRNA2021pt_PT
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