Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/31316
Title: | Step-by-step design of proteins for small molecule interaction: a review on recent milestones |
Author: | Pereira, José M. Vieira, Maria Santos, Sérgio M. |
Keywords: | Protein design Computational chemistry De-novo design Protein synthesis |
Issue Date: | 2-May-2021 |
Publisher: | Wiley |
Abstract: | Protein design is the field of synthetic biology that aims at developing de-novo custom made proteins and peptides for specific applications. Despite exploring an ambitious goal, recent computational advances in both hardware and software technologies have paved the way to high-throughput screening and detailed design of novel folds and improved functionalities. Modern advances in the field of protein design for small molecule targeting are described in this review, organized in a step-by-step fashion: from the conception of a new or upgraded active binding site, to scaffold design, sequence optimization and experimental expression of the custom protein. In each step, contemporary examples are described, and state-of-the art software is briefly explored. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/31316 |
DOI: | 10.1002/pro.4098 |
ISSN: | 0961-8368 |
Publisher Version: | https://onlinelibrary.wiley.com/doi/abs/10.1002/pro.4098 |
Appears in Collections: | CICECO - Artigos DQ - Artigos |
Files in This Item:
File | Description | Size | Format | |
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article.pdf | 5.46 MB | Adobe PDF | View/Open |
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