Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/31102
Title: | Bacterially assembled biopolyester nanobeads for removing cadmium from water |
Author: | Marques, Catarina R. Wibowo, David Rubio-Reyes, Patricia Serafim, Luísa S. Soares, Amadeu M. V. M. Rehm, Bernd H. A. |
Keywords: | Cadmium PHA synthase Metal-binding peptides Biosorption Contact assay |
Issue Date: | 1-Nov-2020 |
Publisher: | Elsevier |
Abstract: | Cadmium (Cd)-contaminated waterbodies are a worldwide concern for the environment, impacting human health. To address the need for efficient, sustainable and cost-effective remediation measures, we developed innovative Cd bioremediation agents by engineering Escherichia coli to assemble poly(3-hydroxybutyric acid) (PHB) beads densely coated with Cd-binding peptides. This was accomplished by translational fusion of Cd-binding peptides to the N- or C-terminus of a PHB synthase that catalyzes PHB synthesis and mediates assembly of Cd2 or Cd1 coated PHB beads, respectively. Cd1 beads showed greater Cd adsorption with 441 nmol Cd mg-1 bead mass when compared to Cd2 beads (334 nmol Cd mg-1 bead-mass) and plain beads (238 nmol Cd mg-1 bead-mass). The Cd beads were not ecotoxic and did attenuate Cd-spiked solutions toxicity. Overall, the bioengineered beads provide a means to remediate Cd-contaminated sites, can be cost-effectively produced at large scale, and offer a biodegradable and safe alternative to synthetic ecotoxic treatments. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/31102 |
DOI: | 10.1016/j.watres.2020.116357 |
ISSN: | 0043-1354 |
Appears in Collections: | CESAM - Artigos CICECO - Artigos DBio - Artigos DQ - Artigos |
Files in This Item:
File | Description | Size | Format | |
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1-s2.0-S0043135420308939-main (2).pdf | 4.37 MB | Adobe PDF | View/Open |
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