Please use this identifier to cite or link to this item:
Title: Cytotoxicity profiling of deep eutectic solvents to human skin cells
Author: Macário, I. P. E.
Oliveira, H.
Menezes, A. C.
Ventura, S. P. M.
Pereira, J. L.
Gonçalves, A. M. M.
Coutinho, J. A. P.
Gonçalves, F. J. M.
Issue Date: 8-Mar-2019
Publisher: Nature Research
Abstract: The tailor-made character of deep eutectic solvents (DES) turns them very attractive to be used in several applications, including in health-related areas such as pharmaceutical, nutraceutical, and cosmetic industries. However, although DES has been touted as "green" solvents, several works proved that their potential toxicity should not be neglected. Using the premise of DES applicability in the cosmetic and pharmaceutical sectors, we chose two cell lines to work as a skin model (keratinocytes HaCaT and tumor melanocytes MNT-1), to assess DES cytotoxicity. The effect of three different hydrogen bond acceptors (HBA) ([Chol]Cl, [N1111]Cl and [N4444]Cl) and three different hydrogen bond donors (HBD) (hexanoic and butanoic acid, ethylene glycol, 1-propanol and urea) were evaluated through a common viability assay (MTT assay). Results were promising since [Chol]Cl and [N1111]Cl- based DES showed good biocompatibility for the tested cells. [N4444]Cl-based DES, however, showed cytotoxicity for both cell lines, with the HBA being the driver of the toxicity. Interestingly, some compounds increased cell viability in the HaCaT cell line, namely [Chol]Cl, ethylene glycol, hexanoic acid, urea, and all [Chol]Cl and [N1111]Cl-based DES and should be considered as targets for future studies. These results highlight their possible use in cosmetic or pharmaceutical formulations.
Peer review: yes
DOI: 10.1038/s41598-019-39910-y
ISSN: 2045-2322
Appears in Collections:CESAM - Artigos
CICECO - Artigos

Files in This Item:
File Description SizeFormat 
s41598-019-39910-y.pdf1.43 MBAdobe PDFView/Open

Formato BibTex MendeleyEndnote Degois 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.