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Title: Understanding prostate cancer cells metabolome: a spectroscopic approach
Author: Santos, Francisco
Magalhães, Sandra
Henriques, Magda Carvalho
Silva, Beatriz
Valença, Isabel
Ribeiro, Daniela
Fardilha, Margarida
Nunes, Alexandra
Keywords: Prostate cancer
Cell lines
Metabolic profile
Principal component analysis
Issue Date: 2018
Publisher: Bentham Science
Abstract: Introduction: Prostate Cancer (PCa) is the second most common neoplasia in men. Because it is often diagnosed at a late stage, mortality rates remain high. Studying cancer metabolome, which reflects early changes that occur in cells, has gained relevance and may contribute to the identification of early diagnostic biomarkers and understanding tumor biology. Methods: Fourier-transform infrared (FTIR) spectroscopy is a metabolomics technique that probes the biochemical composition of the analyzed samples and allows to discriminate samples with distinct metabolic profiles, allowing the discrimination between cancerous and non-cancerous samples. In this study, FTIR spectra were acquired from PCa and normal prostate cell lines and analyzed by Principal Component Analysis (PCA). Results & Conclusion: Our results indicate a clear discrimination between the different cell lines, meaning at they exhibit distinct metabolic profiles. This discrimination can be attributed to an altered lipid metabolism (3000-2800 cm-1, 1800-1700 cm-1 and 1500-1400 cm-1) and changes in protein con-formation (1700-1600 cm-1). These results suggest that studying cancer metabolome with FTIR spec-troscopy not only allows the understanding of tumor metabolic behavior and may be useful to the de-velopment of new therapeutic targets.
Peer review: yes
DOI: 10.2174/2213235X07666181122124106
ISSN: 2213-235X
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