Please use this identifier to cite or link to this item:
http://hdl.handle.net/10773/29287
Title: | Real-time intracellular temperature imaging using lanthanide-bearing polymeric micelles |
Author: | Piñol, Rafael Zeler, Justyna Brites, Carlos D. S. Gu, Yuanyu Téllez, Pedro Carneiro Neto, Albano N. da Silva, Thiago E. Moreno-Loshuertos, Raquel Fernandez-Silva, Patrício Gallego, Ana Isabel Martinez-Lostao, Luis Martínez, Abelardo Carlos, Luís D. Millán, Angel |
Keywords: | Intracellular temperature mapping Luminescence thermometry Lanthanide-based polymer nanomicelles Thermogenesis |
Issue Date: | 9-Sep-2020 |
Publisher: | American Chemical Society |
Abstract: | Measurement of thermogenesis in individual cells is a remarkable challenge due to the complexity of the biochemical environment (such as pH and ionic strength) and to the rapid and yet not well-understood heat transfer mechanisms throughout the cell. Here, we present a unique system for intracellular temperature mapping in a fluorescence microscope (uncertainty of 0.2 K) using rationally designed luminescent Ln3+-bearing polymeric micellar probes (Ln = Sm, Eu) incubated in breast cancer MDA-MB468 cells. Two-dimensional (2D) thermal images recorded increasing the temperature of the cells culture medium between 296 and 304 K shows inhomogeneous intracellular temperature progressions up to ∼20 degrees and subcellular gradients of ∼5 degrees between the nucleolus and the rest of the cell, illustrating the thermogenic activity of the different organelles and highlighting the potential of this tool to study intracellular processes. |
Peer review: | yes |
URI: | http://hdl.handle.net/10773/29287 |
DOI: | 10.1021/acs.nanolett.0c02163 |
ISSN: | 1530-6984 |
Appears in Collections: | CICECO - Artigos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Pinol_10.1021acs.nanolett.0c02163_nanoletters.pdf | 1.17 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.