Improved downstream processing of recombinant proteins using aqueous twophase systems composed of ionic Liquids

Abstract

Previous studies have shown that ionic liquids display highly interesting features concerning protein stabilization, and by properly tailoring their anion/cation pairs, increased selectivity towards the target protein can be achieved in IL-ATPS. Process intensification and scale-up of IL-ATPS for the purification of recombinant proteins can be achieved by centrifugal partition chromatography (CPC), in which the stationary phase is also liquid and kept by centrifugal force.