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|Title:||Aqueous solutions of deep eutectic systems as reaction media for the saccharification and fermentation of hardwood xylan into xylitol|
Morais, Eduarda S.
Soares, Pedro O.
Freire, Mara G.
Freire, Carmen S. R.
Silvestre, Armando J. D.
Simultaneous sacchariﬁcation and fermentation
|Abstract:||The aim of this study was to evaluate the eﬀect of aqueous solutions of deep eutectic solvent, Cholinium Chloride:Urea ([Ch]Cl:U) at50wt%and20 wt%,using diﬀerentmolar ratios(1:1, 2:1and 1:2)onthe enzymatic hydrolysis ofxylanforxyloseproduction anditssubsequent bioconversion into xylitolusing arecombinant yeast strain. The lowest xylan conversion into xylose (45%) was obtained using 1:2 [Ch]Cl:U molar ratio. On the other hand, the 1:1 [Ch]Cl:U molar ratio, at 20 wt% in water, improved this conversion, achieving the highest xylose yield (81.4%). The xylitol production was then optimized with [Ch]Cl:U (1:1) at 20 wt% by simultaneous sacchariﬁcation and fermentation process, attaining 23.67 g/L, corresponding to 66.04% of xylitol yield. This study reveals the possibility of using xylan solubilized in DES aqueous solutions directly for xylitol production, thus assembling a one-step process.|
|Appears in Collections:||CICECO - Artigos|
DQ - Artigos
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